The aim of this study was to develop nanofibrous gelatin substrates for eyelid fat stem cell (EFSC) expansion that can serve as a potential alternative substrate to replace human amniotic membrane. Biocompatibility results indicated that all substrates were highly biocompatible, as EFSCs could favorably attach and proliferate on the nanofibrous surfaces. Microscopic figures showed that the EFSC were firmly anchored to the substrates and were able to retain a normal stem cell phenotype. Immunocytochemistry (ICC) and real time-PCR results revealed change in the expression profile of EFSCs grown on nanofibrous substrates when compared to those grown on control in epithelial induction condition. In addition, electrospun gelatin mats especially oriented scaffold provides not only a milieu supporting EFSCs expansion, but also serves as a useful alternative carrier for ocular surface tissue engineering and could be used as an alternative substrate to amniotic membrane.
The article "ZNF797 plays an oncogenic role in gastric cancer" by D. Momenzadeh, S. Rahman Zadeh, M. Rezaei-Tavirani, A. Baradaran-Rafii, F. Ghasemvand and S. Heidari-Keshel published in Genetics and Molecular Research vol. 13 (4), pp. 8421-8427 in 2014, DOI: http://dx.doi.org/10.4238/2014.October.20.18, has been found to be substantially equal to the article "Role of SALL4 in the progression and metastasis of colorectal cancer" published in the Journal of Biomedical Science, vol. 20, p. 6 in 2013, DOI: 10.1186/1423-0127-20-6, by other authors. The corresponding author of the article published in Genetics and Molecular Research, Saeed Heidari-keshel, alerted our editorial staff about this situation and requested that the article should be retracted. After review and after contacting the authors, the editors of Genetics and Molecular Research have decided to retract the article. The authors and their institutions have been advised of this serious breach of ethics.
ABSTRACT. Human cancer cells resemble stem cells in expression signatures leading them to share some features, most notably, selfrenewal. A complex network of transcription factors and signaling molecules are required for continuation of this trait. ZNF797 (SALL4) is a zinc finger transcriptional activator crucial for maintenance of self-renewal in stem cells; however, its expression level has not yet been elucidated in gastric tumor cells. Its expression was analyzed to determine this level and probable clinicopathological consequences. SALL4 expression in fresh tumor and distant tumor-free tissues from 46 colorectal samples was compared by real-time polymerase chain reaction. Greater than a 2-fold increase in SALL4 expression was detected in 89.5% of tumors vs normal related tissues. SALL4 expression was significantly correlated with tumor cell metastasis to lymph nodes, especially in moderately differentiated tumor samples (P < 0.05). Furthermore, higher levels of SALL4 mRNA expression were significantly associated with younger patients with tumor cells in stages I and II (P < 0.05). These results indicate a relationship between SALL4 expression and tumor cell metastasis to lymph nodes and consequent progression of tumors to advanced stages III and IV. Along with the promising evidence of its role in self-renewal in various cancers, SALL4 is introduced as a potentially interesting therapeutic target to reverse a number of aberrations that promote gastric tumor development and maintenance. This result may lead to new approaches for cancer therapy.
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