Electrospun mat with eyelid fat-derived stem cells as a scaffold for ocular epithelial regeneration

Abstract

The aim of this study was to develop nanofibrous gelatin substrates for eyelid fat stem cell (EFSC) expansion that can serve as a potential alternative substrate to replace human amniotic membrane. Biocompatibility results indicated that all substrates were highly biocompatible, as EFSCs could favorably attach and proliferate on the nanofibrous surfaces. Microscopic figures showed that the EFSC were firmly anchored to the substrates and were able to retain a normal stem cell phenotype. Immunocytochemistry (ICC) and real time-PCR results revealed change in the expression profile of EFSCs grown on nanofibrous substrates when compared to those grown on control in epithelial induction condition. In addition, electrospun gelatin mats especially oriented scaffold provides not only a milieu supporting EFSCs expansion, but also serves as a useful alternative carrier for ocular surface tissue engineering and could be used as an alternative substrate to amniotic membrane.

In vivo assessment of a nanofibrous silk tube as nerve guide for sciatic nerve regeneration

“…Recently, the SF has received an intensive attention as a material for three-dimensional (3 D) porous scaffold because of its excellent intrinsic properties, biocompatibility, biodegradability and mechanical strength [19][20][21][22]. Until now, there are several methods to produce the porous 3D SF scaffolds: freeze-drying, salt-leaching and electrospinning [23][24][25][26][27]. Among those, electrospinning technique has distinct advantages for using as a skin scaffold compared to the conventional 3D scaffolds due to its similarity to extracellular matrix (ECM).…”

Recent developments in regenerative ophthalmology

“…A scaffold composed of electrospun gelatin fiber-alginate gel has also been shown to be mechanically robust and transparent (Tonsomboon and Oyen, 2013). The combination of electrospungelatin mats and eyelid fat stem cells (EFSCs) as a scaffold provided a milieu supporting EFSC expansion and served as a useful alternative carrier in ocular tissue engineering (Momenzadeh et al, 2017). Notably, EFSCs favorably attached and proliferated on nanofibrous surfaces and retained the normal phenotype of stem cells (Momenzadeh et al, 2017).…”

“…The combination of electrospungelatin mats and eyelid fat stem cells (EFSCs) as a scaffold provided a milieu supporting EFSC expansion and served as a useful alternative carrier in ocular tissue engineering (Momenzadeh et al, 2017). Notably, EFSCs favorably attached and proliferated on nanofibrous surfaces and retained the normal phenotype of stem cells (Momenzadeh et al, 2017). Recently, a cell-supporting scaffold composed of gelatin-based photocurable hydrogels was developed for repairing focal corneal wounds .…”

Design considerations and challenges for mechanical stretch bioreactors in tissue engineering

“…For example, in bone–ligament interface regeneration, mesenchymal stem cells seeded in electrospun polylactic co‐glycolic acid scaffolds showed an increase in the metabolic activity with time . In ocular epithelial regeneration, eyelid fat stem cells could firmly anchor to the electrospun gelatin mats and were able to retain a normal stem cell phenotype . Engineered skeletal muscle constructs were prepared from myoblast‐seeded DegraPol® electrospinning microfibrous membranes.…”