The aim of this study was to estimate the glucose diffusion coefficients ex vivo in skin of mice with diabetes induced in vivo by alloxan in comparison to non‐diabetic mice. The temporal dependences of collimated transmittance of tissue samples immersed in glucose solutions were measured in the VIS‐NIR spectral range to quantify the glucose diffusion/permeability coefficients and optical clearing efficiency of mouse skin. The average thickness of intact healthy and diabetic skin was 0.023 ± 0.006 cm and 0.019 ± 0.005 cm, respectively. Considerable differences in optical and kinetic properties of diabetic and non‐diabetic skin were found: clearing efficiency was 1.5‐fold better and glucose diffusivity was 2‐fold slower for diabetic skin. Experimental Setup for measuring collimated transmittance spectra of mouse skin samples.magnified imageExperimental Setup for measuring collimated transmittance spectra of mouse skin samples.

Skin optical clearing can significantly enhance the ability of biomedical optical imaging. Some alcohols and sugars have been selected to be optical clearing agents (OCAs). In this work, we paid attention to the optical clearing potential of disaccharides. Sucrose and maltose were chosen as typical disaccharides to compare with fructose, an excellent monosaccharide-OCA, by using molecular dynamics simulation and an ex vivo experiment. The experimental results indicated that the optical clearing efficacy of skin increases linearly with the concentration for each OCA. Both the theoretical predication and experimental results revealed that the two disaccharides exerted a better optical clearing potential than fructose at the same concentration, and sucrose is optimal. Since maltose has an extremely low saturation concentration, the other two OCAs with saturation concentrations were treated topically on rat skin in vivo, and optical coherence tomography imaging was applied to monitor the optical clearing process. The results demonstrated that sucrose could cause a more significant increase in imaging depth and signal intensity than fructose.

Skin optical clearing effect ex vivo and in vivo was achieved by topical application of low molecular weight paramagnetic magnetic resonance contrast agents. This novel feature has not been explored before. By using collimated transmittance the diffusion coefficients of three clinically used magnetic resonance contrast agents, that is Gadovist, Magnevist and Dotarem as well as X-ray contrast agent Visipaque in mouse skin were determined ex vivo as (4.29 ± 0.39) × 10 −7 cm 2 / s, (5.00 ± 0.72) × 10 −7 cm 2 /s, (3.72 ± 0.67) × 10 −7 cm 2 /s and (1.64 ± 0.18) × 10 −7 cm 2 /s, respectively. The application of gadobutrol (Gadovist) resulted in

In this paper, measurements of the optical properties (diffuse reflectance, total and collimated transmittance) of brain tissues in healthy rats and rats with C6-glioma were performed in the spectral range from 350 to 1800 nm. Using these measurements, characteristic tissue optical parameters, such as absorption coefficient, scattering coefficient, reduced scattering coefficient, and scattering anisotropy factor were reconstructed. It was obtained that the 10-day development of glioma led to increase of absorption coefficient, which was associated with the water content elevation in the tumor. However, further development of the tumor (formation of the necrotic core) led to decrease in the water content. The dependence of the scattering properties on the different stages of model glioma development was more complex. Light penetration depth into the healthy and tumor brain was evaluated.

Abstract. The change of optical parameters of rat skin ex vivo under the action of aqueous 30%, 50%, 60%, 70%, 85%, and 100% glycerol solutions has been experimentally studied. The analysis of kinetics of the change of the studied parameters has allowed the estimate of the diffusion coefficient dependence upon the concentration of glycerol in the skin and the efficiency of optical clearing for each of the glycerol solutions. The results can find application in the development of new methods of noninvasive diagnostics and therapy of skin diseases.

No abstract

In this work, a thorough analysis of hyperosmotic agents for the immersion optical clearing (IOC) in terahertz (THz) range was performed. It was aimed at the selection of agents for the efficient enhancement of penetration depth of THz waves into biological tissues. Pulsed spectroscopy in the frequency range of 0.1 to 2.5 THz was applied for investigation of the optical properties of common IOC agents. Using the collimated transmission spectroscopy in visible range, binary diffusion coefficients of tissue water and agent in ex vivo rat brain tissue were measured. IOC agents were objectively compared using two-dimensional nomogram, accounting for their THz-wave absorption coefficients and binary diffusion coefficients. The results of this study demonstrate an interplay between the penetration depth enhancement and the diffusion rate and allow for pointing out glycerol as an optimal agent among the considered ones for particular applications in THz biophotonics.

Different ways for monitoring the dehydration of tissues affected by hyperosmotic agents have been comparatively analyzed to increase the THz transparency of biological tissues. The data obtained with an original THz laser spectrometer, a Nicolet 6700 Fourier spectrometer, and a Callegari Soft Plus system for skin diagnostics are in good agreement. The corresponding responses of biological tissues (in the form of THz transmittance, reflectance, absorption coefficient, and hydration coefficient) to the effect of biologically compatible hyperosmotic agents have been studied.