THz monitoring of the dehydration of biological tissues affected by hyperosmotic agents

Kolesnikov, Alexandr; Kolesnikova, E. A.; Kolesnikova, K. N.; Tuchina, Daria K.; Попов, А. П.; Skaptsov, Alexander A.; Назаров, М. М.; Shkurinov, A. P.; Terentyuk, Artem G.; Tuchin, Valery V.

doi:10.3103/s1541308x14030029

Cited by 32 publications

(19 citation statements)

References 30 publications

(24 reference statements)

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“…It is important to notice that in vivo application of hyperosmotic agents on the skin causes strong dehydration of the superficial skin layers in a short time period (10‐20 minutes) . At these time intervals, the most part of the agents are concentrated in the epidermis.…”

Section: Resultsmentioning

confidence: 99%

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Sdobnov

Darvin

Schleusener

et al. 2019

Journal of Biophotonics

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Confocal Raman microscopy has been used to measure depth‐dependent profiles of porcine skin ex vivo in the high wavenumber region after application of molecular optical clearing agents (OCAs). Glycerol (70%) and iohexol (100% Omnipaque [300]) water solutions were used as OCAs and topically applied to porcine ear skin for 30 and 60 minutes. Using Gaussian function–based deconvolution, the changes of hydrogen bound water molecule types have been microscopically analyzed down to the depth of 200 μm. Results show that both OCAs induced skin dehydration (reduction of total water), which is 51.3% for glycerol (60 minutes), 33.1% for glycerol (30 minutes), 8.3% for Omnipaque (60 minutes) and 4.4% for Omnipaque (30 minutes), on average for the 40 to 200 μm depths. Among the water types in the skin, the following reduction was observed in concentration of weakly bound (51.1%, 33.2%, 7.5% and 4.6%), strongly bound (50.4%, 33.0%, 7.9% and 3.4%), tightly bound (63.6%, 42.3%, 26.1% and 12.9%) and unbound (55.4%, 28.7%, 10.1% and 5.9%) water types on average for the 40 to 200 μm depths, post application of glycerol (60 minutes), glycerol (30 minutes), Omnipaque (60 minutes) and Omnipaque (30 minutes), respectively. As most concentrated in the skin, weakly and strongly bound water types are preferentially involved in the OCA‐induced water flux in the skin, and thus, are responsible for optical clearing efficiency.

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Section: Resultsmentioning

confidence: 99%

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Sdobnov

Darvin

Schleusener

et al. 2019

Journal of Biophotonics

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“…After obtaining the diffusion data for glucose and water on ex vivo tissues, we are planning to perform measurements in the future on an in vivo animal model to evaluate differences. However, based on our experimental data and direct measurements of tissue hydration for skin, 52 we can predict that tissue physiological reaction (which is delayed due to physiological reaction inertia) will correct the time response on a longer timescale. Nevertheless, we expect similar results to those obtained from ex vivo samples on a shorter time interval (<15 to 20 min).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 93%

Glucose diffusion in colorectal mucosa—a comparative study between normal and cancer tissues

et al. 2017

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Colorectal carcinoma is a major health concern worldwide and its high incidence and mortality require accurate screening methods. Following endoscopic examination, polyps must be removed for histopathological characterization. Aiming to contribute to the improvement of current endoscopy methods of colorectal carcinoma screening or even for future development of laser treatment procedures, we studied the diffusion properties of glucose and water in colorectal healthy and pathological mucosa. These parameters characterize the tissue dehydration and the refractive index matching mechanisms of optical clearing (OC). We used ex vivo tissues to measure the collimated transmittance spectra and thickness during treatments with OC solutions containing glucose in different concentrations. These time dependencies allowed for estimating the diffusion time and diffusion coefficient values of glucose and water in both types of tissues. The measured diffusion times for glucose in healthy and pathological mucosa samples were 299.2 ± 4.7 ?? s and 320.6 ± 10.6 ?? s for 40% and 35% glucose concentrations, respectively. Such a difference indicates a slower glucose diffusion in cancer tissues, which originate from their ability to trap far more glucose than healthy tissues. We have also found a higher free water content in cancerous tissue that is estimated as 64.4% instead of 59.4% for healthy mucosa.

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“…and Kolesnikov et al. found that topical treatment of OCAs (30 % and 40 % glucose, glycerol, propylene glycol, Polyethylene glycol‐600) on human skin could induce different degrees of dehydration, and claimed that the skin transparent window existed from 10 to 22 min after OCA application. In our study, we applied a self‐made reservoir to keep the surface of skin immersed into the abundant reagent, thus the transparent skin window could be maintained for longer times to meet the need of imaging.…”

Section: Discussionmentioning

confidence: 99%

FSOCA‐induced switchable footpad skin optical clearing window for blood flow and cell imaging in vivo

Shi¹,

Feng²,

Zhang³

et al. 2017

Journal of Biophotonics

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The mouse footpad for its feature of hairlessness provides an available window for imaging vascular and cellular structure and function in vivo. Unfortunately, the strong scattering of its skin limits the penetration of light and reduces the imaging contrast and depth. Herein, an innovative footpad skin optical clearing agent (FSOCA) was developed to make the footpad skin transparent quickly by topical application. The results demonstrate that FSOCA treatment not only allowed the cutaneous blood vessels and blood flow distribution to be monitored by laser speckle contrast imaging technique with higher contrast, but also permitted the fluorescent cells to be imaged by laser scanning confocal microscopy with higher fluorescence signal intensity and larger imaging depth. In addition, the physiological saline-treatment could make the footpad skin recover to the initial turbid status, and reclearing would not induce any adverse effects on the distributions and morphologies of blood vessels and cells, which demonstrated a safe and switchable window for biomedical imaging. This switchable footpad skin optical clearing window will be significant for studying blood flow dynamics and cellular immune function in vivo in some vascular and immunological diseases.

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THz monitoring of the dehydration of biological tissues affected by hyperosmotic agents

Cited by 32 publications

References 30 publications

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Glucose diffusion in colorectal mucosa—a comparative study between normal and cancer tissues

FSOCA‐induced switchable footpad skin optical clearing window for blood flow and cell imaging in vivo

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