Two mail surveys were carried out in Denmark and the Eastern Corn Belt, USA in 2002. Questionnaires were sent to 580 farmers who had used precision agriculture (PA) and 198 responses were received. The surveys focused on the current status of use of PA in both countries, including: PA practices, equipment and software, Internet and e-mail use, information sources for PA, satisfaction level from service providers, data handling, interpretation, storage and ownership, value of data for decision making, changes in management practices, desired information and services, and the next planned step in the practice of PA. The survey results showed more similarities in practicing PA between the two countries than differences. Time requirement and high cost of data handling were cited as the main problems. Survey respondents found soil maps to be more valuable than yield maps in management decisions. About 80% of the respondents would like to store the PA data themselves. The majority of the respondents indicated that they have changed their management practices due to PA, but not substantially. Some 90% of the respondents used the Internet and e-mail for agricultural purposes, but only a small number for PA websites.

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Recent outbreaks of food borne illnesses continue to support the need for rapid and sensitive methods for detection of foodborne pathogens. A method for detecting Listeria monocytogenes in food samples was developed using an automated fiber-optic-based immunosensor, RAPTOR ™. Detection of L. monocytogenes in phosphate buffered saline (PBS) was performed to evaluate both static and flow through antibody immobilization methods for capture antibodies in a sandwich assay. Subsequent detection in frankfurter samples was conducted using a flow through immobilization system. A two stage blocking using biotinylated bovine serum albumin (b-BSA) and BSA was effectively employed to reduce the non-specific binding. The sandwich assay using static or flow through mode of antibody immobilization could detect 1×10 3 cfu/ml in PBS. However, the effective disassociation constant K d and the binding valences for static modes of antibody immobilization in spiked PBS samples was 4×10 5 cfu/ml and 4.9 as compared to 7×10 4 cfu/ml and 3.9 for flow through method of antibody immobilization. Thus the sensitive flow-through immobilization method was used to test food samples, which could detect 5×10 5 cfu/ml of L. monocytogenes in frankfurter sample. The responses at the lowest detectable cell numbers in the frankfurter samples was 92.5 ± 14.6 pA for L. monocytogenes to comparative responses of 27.9 ± 12.2 and 31 ± 14.04 pA obtained from Enterococcus Sensors 2006, 6 809 faecalis and Lactobacillus rhamnosus (control species), respectively. The effective K d and binding valency from spiked frankfurter samples was 4.8×10 5 cfu/ml and 3.1, thus showing highly sensitive detection can be achieved using the RAPTOR ™ biosensor even in the presence of other bacterial species in the matrix.

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