In vitro grown neural stem cells from human fetal brain were transplanted to adult rats with spinal trauma. The spinal cord was examined morphologically using histological and immunohistochemical methods on days 5, 15, 30, and 110. Human neural stem/progenitor cells were viable, migrated, and differentiated into neurons and glia in the traumatized spinal cord in adult rats.
Neural stem/progenitor cells from human fetal brain were grown in a tissue culture and transplanted into traumatized spinal cord of adult rats. The behavior and differentiation of transplanted cells were studied morphologically by means of histological and immunohistochemical methods and confocal microscopy. Human neural stem/progenitor cells were viable for not less than 3 months. They migrated and differentiated into neurons and glia in the traumatized spinal cord of adult rats.
In order to study the potentiality of spinal cord reconstruction the experimental work (65 rats) on the selection of the combined biologic graft for stimulation of optimum vascularization, elimination of nerve tissue deficit, restoration of neurologic function and survival rate of animals after dosed contusion of spinal cord was performed. In experimental group (41 rats) suspension of cultured neural stem cells was administered. Control group included 24 animals. For stimulation of compensative restorative processes in the injured spinal cord of adult rats combined grafts with use of either vascularized omentum by Goldsmith or alloaorta (adventitia turned to the spinal cord) with simultaneous of neural stem cells are the most appropriate.
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