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Abstract

In vitro grown neural stem cells from human fetal brain were transplanted to adult rats with spinal trauma. The spinal cord was examined morphologically using histological and immunohistochemical methods on days 5, 15, 30, and 110. Human neural stem/progenitor cells were viable, migrated, and differentiated into neurons and glia in the traumatized spinal cord in adult rats.

Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice

“…Transplants of human brain-derived stem cells or human spinal cord tissue into injured rat spinal cord have been described (7)(8)(9). Moreover, several human cell transplantation paradigms recently have been reported to promote locomotor recovery: human umbilical cell infusion in a rat spinal cord injury model, although only within 3 weeks or less postgrafting (10); neurons differentiated in vitro under retinoic acid from human embryonal teratocarcinoma cells and transplanted into a rat spinal cord injury model (11); human ES cells differentiated in vitro to oligoprogenitors and transplanted into a rat spinal cord injury model (12); and human neural stem͞progenitor cells transplanted into a monkey spinal cord injury model (13).…”

Human Fetal Neural Stem Cells in Rat Brain: Effects of Preculturing and Transplantation

“…Precultured stem/progenitor cells from human fetal brain were transplanted into intact or damaged brain (hypoxic hypoxia), or into mechanically damaged spinal cord of adult rats [1][2][3][4][5]7].…”

“…M. A. Aleksandrova Modern cell technologies stimulate the development of novel methods for the treatment of CNS diseases associated with massive neuronal death. Studies of stem cells are considered as the most promising [4,5]. The search for new methods for stimulation of host cerebral stem cells [7,10] and for transplantation of cultured neural stem/progenitor cells is now in progress [4,9,12,13].…”

“…We studied the effect of the status of recipient brain and the regimen of culturing of the transplanted material on the behavior of human fetal cells transplanted into rat brain [1][2][3][4][5]7,12].…”

“…Studies of stem cells are considered as the most promising [4,5]. The search for new methods for stimulation of host cerebral stem cells [7,10] and for transplantation of cultured neural stem/progenitor cells is now in progress [4,9,12,13].…”