Effect of transplantation of cultured human neural stem and progenitor cells on regeneration of the cornea after chemical burn

Кузнецов, С. Л.; Nikolaeva, L. R.; Спивак, Ю. А.; Chentsova, E. V.; Полтавцева, Р. А.; Marei, M. V.; Сухих, Г. Т.

doi:10.1007/s10517-006-0112-5

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…[2] used cells at a concentration of 1 × 10 6 cells/cm 2 (3 × 10 5 cells/0.2 mL), while Kuznetsov et al. [15] used a concentration of 1 × 10 7 cells/1 mL (2 × 10 6 cells/0.2 mL). In the present study, the concentration of cells used was 1.3 × 10 5 cells/0.2 mL.…”

Section: Discussionmentioning

confidence: 99%

Effects of cultured human adipose‐derived stem cells transplantation on rabbit cornea regeneration after alkaline chemical burn

Lin¹,

Lai²,

Tai³

et al. 2012

The Kaohsiung J of Med Scie

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Ocular chemical burn is a severe injury with poor outcomes. Immediate and appropriate management is highly related to prognosis. We studied the effect of cultured human adipose tissue-derived stem cells on the regeneration of the rabbit cornea after alkaline chemical burn, using used human adipose tissue-derived stem cells as the source material. Immediately after the chemical burn, the experimental eye received a single subconjunctival injection of a stem cell suspension (1.3 × 10(5) cells/0.2 mL), with the other eye serving as control. Rabbits were sacrificed and specimens taken 30 days after injection. The experimental group showed faster wound healing than the control group, and the result for the experimental group was clearer cornea medium. Histologically, there were five to six epithelial cell layers on the corneas of the experimental group as compared to two to three cell layers on the corneas of the control group. Wilcoxon signed rank test showed a significant difference in the epithelial cell layers between the two groups. Surface markers for connexin 43 (Cx43), β-catenin, E-cadherin, and P63 were analyzed. Cx43 and β-catenin showed significant change, as determined by the Wilcoxon signed rank test, which indicated good cell renewal during repair of the corneal epithelium damaged by the chemical burn. E-cadherin and P63 showed no significant change during the epithelium healing process. Transplantation of cultured human adipose tissue-derived stem cells as a treatment for a corneal chemical burn promotes cell renewal and assists in damage repair.

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Section: Discussionmentioning

confidence: 99%

Effects of cultured human adipose‐derived stem cells transplantation on rabbit cornea regeneration after alkaline chemical burn

Lin¹,

Lai²,

Tai³

et al. 2012

The Kaohsiung J of Med Scie

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show abstract

“…Additionally, injuries were induced in the present study with corneal vacuum trephines rather than disks of filter paper as previously used by many researchers [3,4,15,17,21,22]. The reason for this was because the lesions inflicted with the trephines had clearer margins and precisely rounded areas than ones induced by the disks.…”

Section: Discussionmentioning

confidence: 85%

Effects of conditioned media from human amniotic epithelial cells on corneal alkali injuries in rabbits

et al. 2013

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This study was performed to evaluate the effects of conditioned media (CM) from human amniotic epithelial cells (HAECs) on the corneal wound healing process. Eighteen rabbits (36 eyes) were used and randomly assigned to three groups according treatment: CM from HAECs (group 1), vehicle alone (group 2), and saline (group 3). Corneal alkali injuries were induced with 1 N sodium hydroxide. Each reagent used for treatment evaluation was injected into the dorsal bulbar subconjunctiva and the area of the corneal epithelial defect was measured every other day. Two animals from each group were euthanized at a time on days 3, 7, and 15, and the cornea was removed for histological examination. The sum of the epithelial defect areas measured on day 0 to day 6 as well as day 0 to day 14 in group 1 was significantly smaller than those of other groups. Histological examination revealed that the group 1 corneas had less inflammatory cell infiltration and showed more intact epithelial features compared to the other groups. These results suggest that CM from HAECs promote corneal wound healing in rabbits.

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Effect of transplantation of cultured human neural stem and progenitor cells on regeneration of the cornea after chemical burn

Cited by 2 publications

References 5 publications

Effects of cultured human adipose‐derived stem cells transplantation on rabbit cornea regeneration after alkaline chemical burn

Effects of cultured human adipose‐derived stem cells transplantation on rabbit cornea regeneration after alkaline chemical burn

Effects of conditioned media from human amniotic epithelial cells on corneal alkali injuries in rabbits

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