Effects of conditioned media from human amniotic epithelial cells on corneal alkali injuries in rabbits

Kim, Tae Hyun; Park, Young-Woo; Ahn, Jaesang; Ahn, Ji Yeon; Kim, Se-Eun; Jeong, Manbok; Seo, Min-Su; Kang, Kyung‐Sun; Seo, Kangmoon

doi:10.4142/jvs.2013.14.1.61

Cited by 16 publications

(11 citation statements)

References 22 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, some transplanted cells could attenuate myocardial infarction depending on their powerful secretory capacity [ 9 ]. Additionally, hAEC-CM induced the differentiation of human umbilical cord blood mesenchymal stem cells into dopaminergic neuron-like cells [ 10 ] and promoted corneal wound healing in rabbits [ 11 ]. In previous studies, we found that hAECs transplantation increased the fertility of POF/POI mice and restored ovarian function by inhibiting granulose cells apoptosis and alleviating inflammatory reaction in chemo-injured ovaries [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage

Zhang

Sun

et al. 2017

Stem Cell Res Ther

View full text Add to dashboard Cite

BackgroundHuman amniotic epithelial cells (hAECs) are attractive candidates for regenerative medical therapy, with the potential to replace deficient cells and improve functional recovery after injury. Previous studies have demonstrated that transplantation of hAECs effectively alleviate chemotherapy-induced ovarian damage via inhibiting granulose cells apoptosis in animal models of premature ovarian failure/insufficiency (POF/POI). However, the underlying molecular mechanism accounting for hAECs-mediated ovarian function recovery is not fully understood.MethodsTo investigate whether hAECs-secreting cytokines act as molecular basis to attenuate chemotherapy-induced ovarian injury, hAECs or hAEC-conditioned medium (hAEC-CM) was injected into the unilateral ovary of POF/POI mouse. Follicle development was evaluated by H&E staining at 1, 2 months after hAECs or hAEC-CM treatment. In addition, we performed a cytokine array containing 507 human cytokines on hAECs-derived serum-free conditioned medium. Finally, we further investigated whether hAECs could affect chemotherapy-induced apoptosis in primary human granulosa-lutein (hGL) cells and the tube formation of human umbilical vein endothelial cells (hUVECs) via a co-culture system in vitro.ResultsWe observed the existence of healthy and mature follicles in ovaries treated with hAECs or hAEC-CM, whereas seriously fibrosis and many atretic follicles were found in the contralateral untreated ovaries of the same mouse. To distinguish cytokines involved in the process of hAECs-restored ovarian function, hAEC-CM was analyzed with a human cytokines array. Results revealed that 109 cytokines in hAEC-CM might participate in a variety of biological processes including apoptosis, angiogenesis, cell cycle and immune response. In vitro experiments, hAECs significantly inhibited chemotherapy-induced apoptosis and activated TGF-β/Smad signaling pathway within primary granulosa-lutein cells in paracrine manner. Furthermore, hAEC-CM was shown to promote angiogenesis in the injured ovaries and enhance the tube formation of human umbilical vein endothelial cells (hUVECs) in co-culture system.ConclusionsThese findings demonstrated that paracrine might be a key pathway in the process of hAECs-mediating ovarian function recovery in animal models of premature ovarian failure/insufficiency (POF/POI).Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-017-0721-0) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage

Zhang

Sun

et al. 2017

Stem Cell Res Ther

View full text Add to dashboard Cite

show abstract

“…They found IGF (61) , EGF, bFGF, IL-6, and IL-8 (62) in AM-CM and observed an increased proliferation of human corneal epithelial cells when treated with such a CM. Kim et al demonstrated that the use of CM from human AM epithelial cells exerts positive effects on wound healing in an experimental rabbit model with a corneal alkaline injury (63) . These outcomes were considered as a potential alternative for non-surgical treatment in cases of corneal epithelial defects (61)(62)(63) .…”

Section: Therapeutic Use Of Gf/cytokines In Ocular Surface Diseasesmentioning

confidence: 99%

“…Kim et al demonstrated that the use of CM from human AM epithelial cells exerts positive effects on wound healing in an experimental rabbit model with a corneal alkaline injury (63) . These outcomes were considered as a potential alternative for non-surgical treatment in cases of corneal epithelial defects (61)(62)(63) .…”

Section: Therapeutic Use Of Gf/cytokines In Ocular Surface Diseasesmentioning

confidence: 99%

Biological modulation of corneal epithelial wound healing

Loureiro

Gomes

2019

Arquivos Brasileiros De Oftalmologia

View full text Add to dashboard Cite

The transparency and maintenance of corneal epithelial integrity are essential for its optical properties and, to preserve these characteristics, the epithelium undergoes continuous renewal. This renewal depends on the control of cell proliferation and differentiation mediated by mitogenic factors responsible for increasing mitoses and stimulating cellular migration. Cell-cell communication plays a pivotal role in epithelial healing process, and several cytokines and growth factors are involved in this process. Understanding the cross-talk and paracrine effects of these cytokines and growth factors released can help in the search for new therapeutic strategies to treat ocular surface diseases.

show abstract

“…The experimental topical instillation of the secretome in ocular diseases has shown significant improvement in corneal wound healing, attenuating corneal inflammation by inhibition of the proinflammatory cytokines and infiltration of inflammatory cells [ 94 , 95 ]. In allergic conjunctivitis, anti-allergic effects were shown with a reduction of inflammatory cell conjunctival infiltration, inhibition of B cells, mast cells, and histamine functions, through a COX-2-dependent mechanism [ 96 ].…”

Section: Stem Cell Therapymentioning

confidence: 99%

Regenerative Therapies in Dry Eye Disease: From Growth Factors to Cell Therapy

Villatoro

Fernández

Claros

et al. 2017

IJMS

View full text Add to dashboard Cite

Dry eye syndrome is a complex and insidious pathology with a high level of prevalence among the human population and with a consequently high impact on quality of life and economic cost. Currently, its treatment is symptomatic, mainly based on the control of lubrication and inflammation, with significant limitations. Therefore, the latest research is focused on the development of new biological strategies, with the aim of regenerating affected tissues, or at least restricting the progression of the disease, reducing scar tissue, and maintaining corneal transparency. Therapies range from growth factors and cytokines to the use of different cell sources, in particular mesenchymal stem cells, due to their multipotentiality, trophic, and immunomodulatory properties. We will review the state of the art and the latest advances and results of these promising treatments in this pathology.

show abstract

Effects of conditioned media from human amniotic epithelial cells on corneal alkali injuries in rabbits

Cited by 16 publications

References 22 publications

Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage

Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage

Biological modulation of corneal epithelial wound healing

Regenerative Therapies in Dry Eye Disease: From Growth Factors to Cell Therapy

Contact Info

Product

Resources

About