Biological modulation of corneal epithelial wound healing

Loureiro, Renata Ruoco; Gomes, José Álvaro Pereira

doi:10.5935/0004-2749.20190016

Cited by 10 publications

(9 citation statements)

References 56 publications

(77 reference statements)

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“…Cellular reorganization and protein synthesis then occur, with a subsequent loss of the structures responsible for cell adhesion 15 . The cornea then assumes a flattened form and migrates to the wound, thereby covering it 16 . Subsequently, the distal cells then proliferate and migrate to repopulate the wound, which is then followed by cell stratification and differentiation 17 .…”

Section: Discussionmentioning

confidence: 99%

Comparison of rabbit corneal changes during different preservation techniques using optisol-GS and airlift

Tang

Uematsu

Harada

et al. 2023

Sci Rep

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A previous study suggested that the airlift condition is superior to the Optisol-GS condition for preserving the limbal tissue of the human cornea. The purpose of this research is to investigate a new preservation device that preserves the cornea while separating epithelial and endothelial areas. The differences after preserving the corneal epithelium under different conditions were compared. A total of 24 corneas of New Zealand rabbits were divided into four groups in which the corneal epithelia were submersed in Optisol-GS or under airlift conditions for 1 and 2 weeks at 4 $$^{\circ }$$ ∘ C. Transparency, optical coherence tomography (OCT), hematoxylin and eosin (H &E) staining, and epithelial migration tests were used to assess corneal status. The epithelial migration examination showed significantly greater migration ability after the airlift condition. Corneas in the 1-week Optisol-GS group were the most transparent, followed by the 1-week airlift group. OCT showed a progressive increase in corneal thickness to the end of the study. H &E staining showed that the epithelial cells retained intact cellular structure and morphology of the cells for both 1-week-preserved groups. However, there was disruption of the corneal epithelial cell structure for both 2-week-preserved groups. Corneal epithelium preserved under the hypothermic airlift condition was comparable to that under the Optisol-GS condition.

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Section: Discussionmentioning

confidence: 99%

Comparison of rabbit corneal changes during different preservation techniques using optisol-GS and airlift

Tang

Uematsu

Harada

et al. 2023

Sci Rep

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“… 6 , 35 Proper wound repair is vital for corneal epithelial integrity and transparency. 36 , 37 Our work provides new insights into the corneal wound healing process and identifies the IFNβ-MMP13 axis as a potential therapeutic target to promote corneal regeneration.…”

Section: Discussionmentioning

confidence: 95%

dsRNA Induced IFNβ-MMP13 Axis Drives Corneal Wound Healing

Lan

Zhang

Zhu

et al. 2022

Invest. Ophthalmol. Vis. Sci.

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Purpose Cornea, the outermost transparent layer of the eye, is the first line of defense against external threats. Following injury, the wound healing response is crucial to corneal repair and regeneration, yet its underlying mechanism is poorly understood. Our study was designed to investigate the role of dsRNA and its regulatory network in corneal wound healing. Methods A corneal wound healing model was established via the surgical removal of half of the corneal surface and adjoining limbus. RNase III was then used to clarify the role of dsRNA in corneal wound closure and RNA-seq was performed to investigate the mechanism of dsRNA in the healing process. Related gene expression was assessed using immunofluorescence staining, qPCR, and Western blot. Flow cytometry and scratch assay were used to analyze the proliferation and migration of limbal stem/progenitor cells (LSCs) in vitro and functional analysis of the target genes was completed using the corneal wound healing model. Results Corneal wound healing was delayed and impaired when the dsRNAs were removed or damaged following RNase III digestion. The dsRNAs released following corneal damage activate type I interferon (IFN-I) signaling, primarily IFNβ, via the corneal epithelium and neutralizing IFNβ or blocking IFN-I signaling delays corneal wound closure. Moreover, our data identified MMP13 as a downstream effector of IFNβ where its expression promotes LSC proliferation and enhances corneal epithelial reconstruction in vivo. Conclusions The dsRNA induced IFNβ-MMP13 axis plays a key role in corneal wound healing.

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“…Cellular reorganization and protein synthesis then occur, with a subsequent loss of the structures responsible for cell adhesion 12 . The cornea then assumes a flattened form and migrates to the wound, thereby covering it 13 . Subsequently, the distal cells then proliferate and migrate to repopulate the wound, which is then followed by cell stratification and differentiation 14 .…”

Section: Discussionmentioning

confidence: 99%

Comparison of rabbit corneal changes during different preservation techniques using Optisol-GS and airlift.

Tang

Uematsu

Harada

et al. 2023

Preprint

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Background: A previous study has suggested that airlift condition is superior to Optisol-GS condition in preserving the limbal tissue of human cornea. Purpose: To investigate a new preservation device that preserves the cornea while separating epithelial and endothelial areas. We compared the differences after preserving the corneal epithelium in different conditions. Methods: 24 corneas of New Zealand rabbits were divided into 4 groups in which the corneal epithelia submersed in Optisol-GS or under airlift conditions for 1 and 2 weeks, (4°C). Transparency, OCT, H&E staining and epithelial migration tests were used to assess the corneal status. Results: The epithelial migration examination showed statistical difference indicating that the epithelium had larger migration ability after airlift conditions. Corneas in the 1-week Optisol-GS group were the most transparent, followed by the 1-week airlift group. OCT showed progressive increase of corneal thickness till the end of the study. H&E staining results showed that the epithelial cells retained intact cellular structure and morphology of the cells for both the 1-week preserved groups. However, there was disruption of the corneal epithelial cell structure for both 2-week preserved groups. Conclusion: Corneal epithelium preserved under hypothermic airlift conditions was comparable to that found for Optisol-GS conditions.

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Biological modulation of corneal epithelial wound healing

Cited by 10 publications

References 56 publications

Comparison of rabbit corneal changes during different preservation techniques using optisol-GS and airlift

Comparison of rabbit corneal changes during different preservation techniques using optisol-GS and airlift

dsRNA Induced IFNβ-MMP13 Axis Drives Corneal Wound Healing

Comparison of rabbit corneal changes during different preservation techniques using Optisol-GS and airlift.

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