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SUMMARYCell walls of Prototheca zopJii KRUGER 236/65 were prepared by differential centrifugation after disruption of the organisms in a French press. The isolated walls were free from whole organisms and subcellular particles as shown by examination by light and electron microscopy. A quantitative analysis of the walls was made with respect to total nitrogen, phosphorus, ash and lipid. Paper chromatography of hydrolysates of walls showed glucose, mannose, glucosamine and twelve amino acids. A quantitative amino acid analysis was made by ion-exchange chromatography; trace amounts of several other amino acids were detected. The structure of the walls was studied before and after treatment with NaOH and a variety of hydrolytic enzymes. The results are compared with those obtained by other workers with various species of Chlorella, Aspergillus and yeasts. The results are discussed with reference to the taxonomic position of Prototheca zopjii.

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