Calcium displacement by local anesthetics. Dependence on pH and anesthetic charge.

Low, Philip S.; Lloyd, D.; Stein, Thomas M.; Rogers, Jane

doi:10.1016/s0021-9258(18)50705-5

Cited by 127 publications

(3 citation statements)

References 48 publications

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“…The effect of La ... on fluorescence may be the result of a net efflux of Ca" from the ER into nonmembrane cytoplasm, into the mitochondria and plastids, or into the external medium . Perfusion with EGTA, a strong Ca" chelator, or with procaine, an anesthetic that modifies the Ca" distribution in sarcoplasmic reticulum (37), causes a marked reduction in diffuse fluorescence, without modifying punctate CTC fluorescence in endosperm cells. These treatments suggest that the Ca" giving rise to the diffuse fluorescence is more easily exchanged than that in mitochondria and plastids, although this result may merely reflect significant differences in total Ca++ content in various cytoplasmic membrane compartments .…”

Section: Tile Journal Of Cell Biology -Volume 87 1980mentioning

confidence: 99%

Detection of the membrane-calcium distribution during mitosis in Haemanthus endosperm with chlorotetracycline.

Wolniak¹,

Hepler²,

Jackson³

1980

The Journal of Cell Biology

137

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The distribution of membrane-associated calcium has been determined at various stages of mitosis in Haemanthus endosperm cells with the fluorescent chelate probe chlorotetracycline (CTC) . CTC fluorescence in Haemanthus has two components : punctate, because of mitochondrial and plastid membrane-Ca" ; and diffuse, primarily because of Ca" associated with endoplasmic reticulum membranes. Punctate fluorescence assumes a polar distribution throughout mitosis. Cones of diffuse fluorescence in the chromosome-to-pole regions of the metaphase spindle appear to coincide with the kinetochore fibers ; during anaphase, the cones of fluorescence coalesce and this region of the spindle exhibits uniform diffuse fluorescence. Perturbation of the cellular Ca" distribution by treatment with lanthanum, procaine, or EGTA results in a loss of diffuse fluorescence with no accompanying change in the intensity of punctate fluorescence . Detergent extraction of cellular membranes causes a total elimination of CTC fluorescence . CTC fluorescence of freshly teased crayfish claw muscle sarcoplasmic reticulum coincides with the A bands and is reduced by perfusion with lanthanum, procaine, and EGTA in a manner similar to that for diffuse fluorescence in the endosperm cells. These results are consistent with the hypothesis that a membrane system in the chromosome-to-pole region of the mitotic apparatus functions in the localized release of sequestered Ca", thereby regulating the mechanochemical events of mitosis .The formation and operation of the mitotic apparatus implies the presence of a system to regulate the assembly and function of motile elements responsible for chromosome movement. Although great effort has been directed toward determining the distribution and chemical nature ofmotile elements of the mitotic apparatus, such as tubulin (20,31,39) and actin (21,22), the distribution and functions ofmembranes in the spindle have received little attention until recently (25)(26)(27)(28).One of the plausible roles for a membrane system in the mitotic apparatus is the sequestration and localized release of calcium (26,27) . Calcium exerts profound effects on the stability of spindle microtubules both in vivo (32, 33) and in vitro (40), as well as on chromosome displacement in vitro (40), an effect that may be important in the regulation ofmitotic events . Recent studies have demonstrated the redistribution of cytoplasmic membranes (the nuclear envelope-endoplasmic reticulum [NE-ER] complex) during mitosis in fixed cells ofbarley

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Section: Tile Journal Of Cell Biology -Volume 87 1980mentioning

confidence: 99%

Detection of the membrane-calcium distribution during mitosis in Haemanthus endosperm with chlorotetracycline.

Wolniak¹,

Hepler²,

Jackson³

1980

The Journal of Cell Biology

137

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“…Calcium ions added to membrane preparations are known to increase phospholipid order (Seeman, 1972), in a competitive manner with CPZ (Breton et al, 1977). On the other hand, local anesthetics, regardless of charge, including tetracaine and straight-chain alcohols, displace Ca2+ from the cytoplasmic face of membranes (Low et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

Modulation of adenylate cyclase activity by the physical state of pigeon erythrocyte membrane: 1. Parallel drug-induced changes in the bilayer fluidity and adenylate cyclase activity

Salesse¹,

Garnier²,

Leterrier³

et al. 1982

Biochemistry

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The fluorescence anisotropy probe perylene and the spin-labels 5-doxylsterate and 16-doxylstearate were used to estimate the order and internal microviscosity of the pigeon erythrocyte membrane upon perturbation by cationic or neutral amphipathic drugs (chlorpromazine, methochlorpromazine, tetracaine, and octanol) and an anionic drug, octanoic acid. Both methods gave identical results. The fluidity changes were found to strictly correlate with those of adenylate cyclase activity in the presence of GTP when perturbed by the drugs [Salesse, R., & Garnier, J. (1979) Biochim. Biophys. Acta 554, 102-113]. The cationic or neutral drugs, in an intermediate range of concentration, decreased the degree of organization and the internal microviscosity of the lipids together with the activity of the adenylate cyclase. At a higher concentration they reincreased them up to or higher than their initial level before the final destruction of the membrane structure and functions. This concentration effect was time dependent with tetracaine. The quaternary amine methochlorpromazine acted as chlorpromazine only on open ghosts. On intact cells, it inhibited catecholamine receptors at higher concentration and monotonously decreased the order and microviscosity, as the anionic amphipath octanoic acid did. This is taken as evidence that the inner leaflet of the bilayer is the seat for the observed multiphasic changes of viscosity and the control of adenylate cyclase and catecholamine receptors. This could stem from either a preferential intercalation or a surface effect of the amphipaths in the inner leaflet of the membrane. Since the basal activity of adenylate cyclase was not affected in the presence of drugs, it may be inferred that the enzyme holds its activity but that its stimulation is modulated by the membrane physical state.

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“…Οι διαφορές, μπορεί κάλλιστα να συγκριθούν μ' αυτές που παρατηρούνται στα μεθυλο-ή αιθυλο-παράγωγα των αντίστοι χων τριτοταγών αμινών. Έτσι, η διαφορά της τριμεθυλαμίνης (pka 9,76) από την Ν-μεθυλοδιαιθυλαμίνη (pka 10,29) είναι +0>53 ενώ στα αντίστοιχα Ν-αιθυλοπαράγωγα η Äpka είναι +0,66 [176] 38). Και στις δύο πε ριπτώσεις τα πυρροΛιδινο-παράγωγα φαίνονται να είναι πιο ισ χυρές βάσεις από τα πιπεριδινο-και η διαφορά σαφώς είναι πιο αισθητή στην περίπτωση των Ν-μεθυΛοπαραγώγων.…”

Section: σταθερα ιονισμουunclassified

Φαρμακοχημικη Μελετη Νεων Αμινοκετονων: Συνθεση Φυσικοχημικες Ιδιοτητες Τοπικη Αναισθητικη Δραση. Συσχετισεις Δομης-Δρασης

Σωτηροπουλου¹

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ΠΡΟΛΟΓΟΣ Η παρούσα διατριβή έγ uve εΐ ολοκλήρου στο εργαστήριο της Φαρμακευτικής Χημείας του Αριστοτελείου Πανεπιστημίου θεσσα λονίκης. θεωρώ υποχρέωση μου να εκφράσω τις Θερμές ευχαριστίες μου στον διευθυντή του Τομέα της Φαρμακευτικής Χημείας καθηγητή κ. Π.Ν. Κουρουνάκη, ο οποίος μου υπέδειΊε το θέμα της διατριβής και ως επιβλέπων της τριμελούς συμβουλευτικής επιτροπής, συ νέβαλε ουσιαστικά στην εκπόνηση, ολοκλήρωση και αρτιότερη εμ φάνιση της. Τον επίκ. καθηγητή Β. Δημόπουλο, μέλος της τριμελούς συμ βουλευτικής επιτροπής, ευχαριστώ για την βοήθεια του και τις εποικοδομητικές παρατηρήσεις του κο:τά την διόρθωση του τελικού κειμένου. Την λέκτορα Α. Γερονικάκη, μέλος της τριμελούς συμβουλευ τικής επιτροπής, ευχαριστώ θερμά για την βοήθεια της κατά την διάρκεια της εκπόνησης και την ουσιαστική συμβολή της στην αρτιότερη εμφάνιση της παρούσας διατριβής. Τον αναπληρωτή καθηγητή του τμήματος Βιολογίας κ. Γ. θεοφιλλίδη, ευχαριστώ για την ουσιαστική του βοήθεια στη Πει ραματική Διαδικασία του Βιολογικού Μέρους της διατριβής. Τους λέκτορες του Τομέα της Φαρμακευτικής Χημείας, Ε. Ρέκκα και Δ. Χατζηπαύλου, ευχαριστώ για τις εύστοχες υποδεί ξεις και παρατηρήσεις τους. Την παρασκευάστρια του Τομέα Φαρμακευτικής Χημείας Μ. Τόμη-Χατζή ευχαριστώ θερμά για την επιμέλεια των σχημάτων και την τεχνική βοήθεια. Τους παρασκευαστές του Τμήματος Χημείας, Γ. Μπαρμπαράτσα και Ε. Ποχιλίδου, ευχαριστώ για την λήψη μέρους των στοιχεια κών αναλύσεων και Δ. Ρήγα για τη λήψη των φασμάτων μά"ζρς. Τέλος ευχαριστώ την οικογένεια μου για την αμέριστη συ μπαράσταση και ουσιαστική βοήθεια της, στην οποία με χαρά αφιερώνω την παρούσα διατριβή.

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Calcium displacement by local anesthetics. Dependence on pH and anesthetic charge.

Cited by 127 publications

References 48 publications

Detection of the membrane-calcium distribution during mitosis in Haemanthus endosperm with chlorotetracycline.

Detection of the membrane-calcium distribution during mitosis in Haemanthus endosperm with chlorotetracycline.

Modulation of adenylate cyclase activity by the physical state of pigeon erythrocyte membrane: 1. Parallel drug-induced changes in the bilayer fluidity and adenylate cyclase activity

Φαρμακοχημικη Μελετη Νεων Αμινοκετονων: Συνθεση Φυσικοχημικες Ιδιοτητες Τοπικη Αναισθητικη Δραση. Συσχετισεις Δομης-Δρασης

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