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It was shown that the studied petroleum products, kerosene and gasoline, contain microfloccules of heterogeneous microbial biofilms, the cells of which are integrated in a polymer matrix containing acidic polysaccharides. Thirteen bacterial strains were microbiologically isolated from petroleum products, and their taxonomy was identified via analysis of the 16S rRNA sequence. Kerosene was characterized by a diverse bacterial composition, including the following genera: Sphingobacterium, Alcaligenes, Rhodococcus, and Deinococcus. The gasoline bacterial community included only two genera: Bacillus and Paenibacillus. Representatives of the Deinococcus genus that are capable of growing on hydrocarbons were isolated from fuels for the first time. Strains isolated from gasoline (Bacillus safensis Bi13 and Bacillus sp. Bi14) proved to be the most effective biodegraders of all n-alkanes, isoalkanes, cycloalkanes, alkenes, and aromatic hydrocarbons, whereas the strain Rhodococcus erythropolis Bi6, which was isolated from kerosene, effectively decomposed only n-alkanes and trimethylbenzene. Both types of the studied petroleum products contained hydrocarbonoxidizing communities, some members of which were more active in hydrocarbon biodegradation, while others were capable of producing biosurfactants and compounds with emulsifying activity (Deinococcus sp. Bi7) or had increased (well above average) cell-wall hydrophobicity (Sphingobacterium sp. Bi5 from kerosene; Bacillus pumilus Bi12 from gasoline). The indicated properties of the studied strains make them promising for use in bioremediation.

A consortium of microorganisms were isolated from TC-1 fuel form, each member of which is capable of consistently degrade hydrocarbons’ different fractions. The 5 strains of hydrocarbon-oxidizing bacteria (PSB) were identified and isolated from TS-1 jet fuel. Their physiological and biochemical features are defined. All strains exhibit positive catalase activity. It is determined that all UOB strains, producing exogenous and endogenous surfactants, are capable to growth on media with different fraction of hydrocarbons. The study of these associations allows to create effective preparations for bioremediation in the elimination of accidental spills of oil and petroleum products.

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It has been shown that the studied petroleum products (kerosene and gasoline) contain microflocules of heterogeneous microbial biofilms, the cells of which are integrated into a polymer matrix containing acidic polysaccharides. Thirteen bacterial strains were microbiologically isolated from petroleum products, and their taxonomy was identified by the 16S rRNA sequence. Kerosene was characterized by a diverse bacterial composition including the following genera: Sphingobacterium, Alcaligenes, Rhodococcus and Deinococcus, while gasoline bacterial community included only two genera: Bacillus and Paenibacillus. Representatives of the Deinococcus genera capable of growing on the hydrocarbons were isolated from fuels for the first time. The strains isolated from gasoline (Bacillus safensis Bi13 and Bacillus sp. Bi14) proved to be the most effective biodegraders of all n-alkanes, isoalkanes, cycloalkanes, alkenes and aromatic hydrocarbons, whereas the kerosene strain Rhodococcus erythropolis Bi6 effectively decomposed n-alkanes and trimethylbenzene. Both types of petroleum products contained hydrocarbon-oxidizing communities, some members of which were more active in the biodegradation of hydrocarbons, while others were capable of producing biosurfactants and had either emulsifying activity (Deinococcus sp. Bi7) or cell wall hydrophobicity (Sphingobacterium sp. Bi5 from kerosene; Bacillus pumilus Bi12 from gasoline) significantly higher than the average level. The indicated properties of the studied strains make them promising for use in bioremediation. biodegradation, petroleum products, hydrocarbon-oxidizing bacteria, bio-surfactants The work was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation (topic no. 10.5422.2017/8.9.). Investigation of microbial potential in the use hydrocarbons was supported by the Russian Foundation for Basic Research (RFBR), contract no. 18-29-05067. Physicochemical research was performed within the framework of the state assignment to the TIPS RAS