Microorganisms in spent water-miscible metalworking fluids as a resource of strains for their disposal

Elansky, S. N.; Chudinova, Elena M.; Elansky, Alexander S.; Kah, Masamba O.; Sandzhieva, D. A.; Mukabenova, Bayana A.; Дедов, А. В.

doi:10.1016/j.jclepro.2022.131438

Cited by 9 publications

(8 citation statements)

References 25 publications

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“…The method of CTAB extraction was used to extract DNA from fungal cultures. After 5-7 days of incubation at 18°C, the mycelium was separated from the liquid pea medium, dried about 30 min on a lter paper at room temperature, and used for DNA isolation (Elansky et al, 2022). About 0,25 ml of mycelium were added directly to 700 µl extraction buffer (200 mM Tris HC1 pH 8.5, 250 mM NaCl, 25 mM EDTA, 2% CTAB) in a 1.5 ml Eppendorf tube.…”

Section: Dna Extractionmentioning

confidence: 99%

Assessing boscalid efficacy and resistance of large-spored Alternaria pathogens of potato and tomato crops

Kokaeva,

Berezov,

Pobedinskaya

et al. 2024

Preprint

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Large-spored Alternaria species of the section Porri were evaluated for resistance to boscalid through laboratory assays. Most isolates had EC50 values above 100 µg/mL, indicating widespread fungicide resistance. Only isolates of A. solani demonstrated sensitivity with EC50 values below 7 µg/mL. Isolates of A. linariae, A. protenta and A. alternariacida were resistant. A moderate level of resistance to the fungicide was found in A. grandis (mean EC50 = 76,8 µg/mL). Even though the resistance levels varied among strains, sampling location did not appear to be a significant factor. One of the primary targets of boscalid within fungal cells is the succinate dehydrogenase (SDH) enzyme complex, which consists of several subunits. Mutations in these genes have been identified to be a key mechanism of boscalid resistance in various fungal pathogens. Our study revealed the absence of H134R and H278Y mutations within the SdhB gene among the Alternaria isolates. We further analyzed the sequences from Sdh gene subunits B, C, and D in the isolates originating from potato, including A. alternariacida, A. grandis, A. linariae, A. protenta, and A. solani. This analysis identified several single nucleotide polymorphisms (SNPs) that effectively distinguished these isolates from the tomato-derived A. linariae ones.

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Section: Dna Extractionmentioning

confidence: 99%

Assessing boscalid efficacy and resistance of large-spored Alternaria pathogens of potato and tomato crops

Kokaeva,

Berezov,

Pobedinskaya

et al. 2024

Preprint

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“…Despite the efforts to extend the end life of MWFs as much as possible, the manufacturing sector annually produces 20 million tons of spent MWFs (SMWFs), 14 which require a proper treatment before disposal to avoid serious sanitary and environmental risks. However, a single MWF may contain up to 60 of the >300 compounds generally used during its production process 13 .…”

Section: Introductionmentioning

confidence: 99%

“…However, SMWF biological treatments are generally hindered by the presence of biocides and other compounds that inhibit microbial activity 15 . Then, one of the most interesting approaches consists of exploiting the SMWF indigenous microbial community to decrease both the biochemical oxygen demand (BOD) and chemical oxygen demand (COD) of the solution 14,16 . Notably, microbial communities properly selected and enriched from specific environments can display better acclimatization and contaminant biodegradation capacities than conventional ones 17,18 .…”

Section: Introductionmentioning

confidence: 99%

“…15 Then, one of the most interesting approaches consists of exploiting the SMWF indigenous microbial community to decrease both the biochemical oxygen demand (BOD) and chemical oxygen demand (COD) of the solution. 14,16 Notably, microbial communities properly selected and enriched from specific environments can display better acclimatization and contaminant biodegradation capacities than conventional ones. 17,18 However, the selection of these communities, with higher resistance to the biocides in SMWFs, may also lead to the release of antimicrobial-resistant genes in the environment, thus making this type of treatment a potential source of these genes in environmental microbiomes.…”

Section: Introductionmentioning

confidence: 99%

“…12 In addition, MWFs may contain other potentially toxic compounds such as emulsifiers, coupling agents, corrosion inhibitors, extreme-pressure additives, biocides, and antioxidants, which are used as additives to both increase productivity and reduce the aging and deterioration rate of the fluid. 13 Despite the efforts to extend the end life of MWFs as much as possible, the manufacturing sector annually produces 20 million tons of spent MWFs (SMWFs), 14 which require a proper treatment before disposal to avoid serious sanitary and environmental risks. However, a single MWF may contain up to 60 of the >300 compounds generally used during its production process.…”

Section: Introductionmentioning

confidence: 99%

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Pilot‐scale treatment cycle comprising membrane, thermal, and adsorption processes for spent metalworking fluids remediation

Capua

Ferraro

Lepore³

et al. 2023

J of Chemical Tech & Biotech

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BACKGROUND Spent metalworking fluids (SMWFs) represent a process waste characterized by low biodegradability, high toxicity, and very heterogeneous contamination typology connoted by the occurrence of numerous different pollutants. In this regard, the development of technically flexible and efficient treatment systems is fundamental to prevent sanitary and environmental risks deriving from SMWF disposal. RESULTS A treatment cycle including ultrafiltration (UF), evaporation, reverse osmosis (RO), and granular activated carbon (GAC) adsorption was tested at pilot scale for the remediation of three real SMWFs. A total of 44 parameters (physical, chemical, and ecotoxicological), identifying the SMWF characteristics and contamination, were analyzed after each treatment unit. The treatment cycle performance was investigated with and without the UF phase. In the complete cycle, UF was effective for the decrease of (average values) total suspended solids (96%), total hydrocarbons (>99%), animal fats and oils (>99%), and ecotoxicity (85%). In the same cycle, the evaporation significantly reduced the organic content (91% on average), and RO followed by GAC adsorption further provided effluents suitable for reuse in industrial applications. For the cycle without UF, parameters such as chemical oxygen demand, biochemical oxygen demand, and ecotoxicity showed values still significant (on average equal to 5550 mg L−1, 1933 mg L−1, and 95%, respectively) after evaporation. However, RO and GAC adsorption units allowed the achievement of effluents suitable for discharge in sewer systems. CONCLUSIONS According to the results, this work provides treatment strategies displaying high SMWF remediation efficiency and wide adaptability over various requirements for the achievement of specific effluent quality standards. © 2023 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

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A monosurfactant-stabilized dual-responsive and versatile emulsion lubricant

Hou

Yu²,

Chen

et al. 2023

Journal of Cleaner Production

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Microorganisms in spent water-miscible metalworking fluids as a resource of strains for their disposal

Cited by 9 publications

References 25 publications

Assessing boscalid efficacy and resistance of large-spored Alternaria pathogens of potato and tomato crops

Assessing boscalid efficacy and resistance of large-spored Alternaria pathogens of potato and tomato crops

Pilot‐scale treatment cycle comprising membrane, thermal, and adsorption processes for spent metalworking fluids remediation

A monosurfactant-stabilized dual-responsive and versatile emulsion lubricant

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