Virgin Coconut oil (VCO), owing to its functional properties (important in , is costly and, therefore, susceptible to adulteration with other cheaper oils like coconut oil. An enzyme-based biosensor confirmative test of VCO was constructed by co-immobilizing enzymes onto a glassy carbon electrode. The performance of the biosensor was optimized at a potential of +0.5 V with 45 mg gelatin, 30 mg BSA coupled with 2.5% glutaraldehyde at pH 7.0 with an incubation time of 1 hr. Adulterated samples of VCO with coconut oil (CO) were analyzed. The concentration of diglyceride (DG) was estimated from the empirical relation, which showed a linear increase with the increase in adulteration. The developed biosensor was validated using highperformance liquid chromatography (HPLC) methods using a paired sample t test at a 5% significance level. The biosensor could detect adulteration in VCO with CO above 20% within 3-5 s and can be reused for 25 days.
Practical applicationsAdulteration of edible oils is a massive issue in today's market. Virgin coconut oil due to its functional and antiviral properties becomes important for human health. This study revealed that the developed biosensor could be an effective method for adulteration detection in virgin coconut oil with coconut oil.
This study aims to compare the physicochemical properties of Virgin Coconut Oil (VCO), Refined, Bleached and Deodorized (RBD) oil and Cold Pressed Oil (CPO, Chekku oil). The physicochemical properties taken for the study includes moisture content, refractive index, saponification value, iodine value, acid value, unsaponifiable matter, polenske value and % free fatty acids. The physicochemical properties of all three samples obtained are in compliance with Asian and Pacific Coconut Community (APCC) standards. Of all the samples, VCO showed 1.3% FFA which was higher when compared, that it should be consumed only to a certain limit for cooking. VCO has very low moisture content of 0.11% where the shelf life of oil can be naturally preserved. The acid value of VCO was 2.7 mg KOH/g which has the highest amount of AV whereas for other samples it was less than 1.0 mg KOH/g. These findings can be used for chemical analysis and adulteration detection.
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