Abstract:Virgin Coconut oil (VCO), owing to its functional properties (important in , is costly and, therefore, susceptible to adulteration with other cheaper oils like coconut oil. An enzyme-based biosensor confirmative test of VCO was constructed by co-immobilizing enzymes onto a glassy carbon electrode. The performance of the biosensor was optimized at a potential of +0.5 V with 45 mg gelatin, 30 mg BSA coupled with 2.5% glutaraldehyde at pH 7.0 with an incubation time of 1 hr. Adulterated samples of VCO with coconu… Show more
“…In addition, many other enzyme-based biosensors have been developed using the method of crosslinked gelatin immobilization. These biosensors present the potential for the detection of citric acid ( Figure 2 b) [ 45 ], sulfite [ 46 , 47 ] and diglyceride ( Figure 2 c) [ 48 ]. Considering its good biosafety and edibility, gelatin is a good matrix for immobilizing biosensing materials for developing biosensors.…”
Section: Gelatin As a Matrix For Immobilized Biorecognition Materialsmentioning
confidence: 99%
“… Figure 2 Biosensors for food testing with gelatin matrix. ( a ) Schematic representation of the screen-printed electrode biosensor [ 44 ] (copyright (2020) D. Manoj et al); ( b ) red markings point the steps that pose potential challenge towards ensuring the sensory and nutritional quality of the fruit juice [ 45 ] (copyright (2022) Elsevier Ltd.); ( c ) amperometric enzyme-based biosensor to evaluate adulteration in virgin coconut oil [ 48 ] (copyright (2022) Wiley Periodicals LLC. ); ( d ) scheme of fabrication procedure for electrospun Hb/gelatin-MWCNTs/GC electrode [ 50 ] (copyright (2020) Elsevier B.V.).…”
Section: Gelatin As a Matrix For Immobilized Biorecognition Materialsmentioning
Gelatin is a natural protein from animal tissue with excellent biocompatibility, biodegradability, biosafety, low cost, and sol–gel property. By taking advantage of these properties, gelatin is considered to be an ideal component for the fabrication of biosensors. In recent years, biosensors with gelatin have been widely used for detecting various analytes, such as glucose, hydrogen peroxide, urea, amino acids, and pesticides, in the fields of medical diagnosis, food testing, and environmental monitoring. This perspective is an overview of the most recent trends and progress in the development of gelatin-based biosensors, which are classified by the function of gelatin as a matrix for immobilized biorecognition materials or as a biorecognition material for detecting target analytes.
“…In addition, many other enzyme-based biosensors have been developed using the method of crosslinked gelatin immobilization. These biosensors present the potential for the detection of citric acid ( Figure 2 b) [ 45 ], sulfite [ 46 , 47 ] and diglyceride ( Figure 2 c) [ 48 ]. Considering its good biosafety and edibility, gelatin is a good matrix for immobilizing biosensing materials for developing biosensors.…”
Section: Gelatin As a Matrix For Immobilized Biorecognition Materialsmentioning
confidence: 99%
“… Figure 2 Biosensors for food testing with gelatin matrix. ( a ) Schematic representation of the screen-printed electrode biosensor [ 44 ] (copyright (2020) D. Manoj et al); ( b ) red markings point the steps that pose potential challenge towards ensuring the sensory and nutritional quality of the fruit juice [ 45 ] (copyright (2022) Elsevier Ltd.); ( c ) amperometric enzyme-based biosensor to evaluate adulteration in virgin coconut oil [ 48 ] (copyright (2022) Wiley Periodicals LLC. ); ( d ) scheme of fabrication procedure for electrospun Hb/gelatin-MWCNTs/GC electrode [ 50 ] (copyright (2020) Elsevier B.V.).…”
Section: Gelatin As a Matrix For Immobilized Biorecognition Materialsmentioning
Gelatin is a natural protein from animal tissue with excellent biocompatibility, biodegradability, biosafety, low cost, and sol–gel property. By taking advantage of these properties, gelatin is considered to be an ideal component for the fabrication of biosensors. In recent years, biosensors with gelatin have been widely used for detecting various analytes, such as glucose, hydrogen peroxide, urea, amino acids, and pesticides, in the fields of medical diagnosis, food testing, and environmental monitoring. This perspective is an overview of the most recent trends and progress in the development of gelatin-based biosensors, which are classified by the function of gelatin as a matrix for immobilized biorecognition materials or as a biorecognition material for detecting target analytes.
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