Isobaric tagging using reagents such as tandem mass tags (TMT) and isobaric tags for relative and absolute quantification (iTRAQ) have become popular tools for mass spectrometry based quantitative proteomics. Because the peptide quantification information is collected in tandem mass spectra, the accuracy and precision of this method largely depend on the resolution with which precursor ions can be selected for the fragmentation and the specificity of the generated reporter ion. The latter can constitute an issue if near isobaric ion signals are present in such spectra because they may distort quantification results. We propose a simple remedy for this problem by identifying reporter ions via the accurate mass differences within a single tandem mass spectrum instead of applying fixed mass error tolerances for all tandem mass spectra. Our results show that this leads to unambiguous reporter ion identification and complete removal of interfering signals. This mode of data processing is easily implemented in software and offers advantages for protein quantification based on few peptides.
Purpose-The study examines Millennials' formation of trust towards a travel website and identifies the similarities and differences in trust formation among consumers from two countries-Denmark and Portugal. Design/methodology/approach-The study is based on online surveys conducted with convenience samples from two culturally distant countries. Independent t-tests, structural equation modelling and multi-group analysis are used to verify the conceptual model and test the hypotheses. Findings-Results support a strong relationship between initial trust towards a travel website and consumers' behavioural intentions. The results also suggest that cultural differences between countries moderate the formation of initial trust and behavioural reactions hereto. Originality/value-The study provides new insights into understanding how Millennials from Portugal and Denmark form initial trust towards an e-travel website.
Es ist eine der Aufgaben des naturwissenschaftlichen Unterrichts, Schülern Techniken nahezubringen, die im Alltag eine wichtige Rolle spielen. Ein Beispiel sind moderne elektrochemische Speichermedien.
The reception of the legend of Arthur in the Tudor era presents something of a paradox. On the one hand, Arthur featured prominently in pageants and public spectacles throughout the period, and at times played a surprisingly important role in foreign policy. On the other hand, chroniclers found it increasingly difficult to defend Arthur’s historicity, and the period failed to produce a major work of Arthurian literature beyond Spenser’s Faerie Queene, in which the British prince cuts a perplexingly elusive figure. With its complex and conflicting attitudes to the Arthurian tradition, the Tudor era seems to constitute a bridge or way-station between the Arthur of the Middle Ages and the Arthur of more securely post-medieval (and, hence, medievalist) eras.
Eternal Shadow of the finite Soul, The Soul's self-symbol, its image of itself.Its own yet not itself--(Fragment, ST Coleridge, undated [1810?]) In the history of ideas about the relationship between mind and body, On the issue of human identity, these sciences share a foundational premise, have comparable aims and have adopted similar methodological approaches.These sciences conceive of the body as intimately linked to, indeed inseparable from, the mind and/or the 'self.' They are underwritten by the belief that the body shapes-if not determines-character, behaviour and intelligence. From this premise, it follows that the body conveys information about such things as pathology, moral depravity, sexual deviance and criminal predisposition. There is great diversity between these sciences and within their specialized branches, yet
The modern history of machines that mimic humans -automata, artist's dummies, mannequins, mechanical dolls, poupées, robots, androids, bionic men and women -is long and varied. Since the birth of the Enlightenment, these adaptable machines have been a testing ground for that perennial question: what does it mean to be human? Eighteenthcentury varieties reflected the rise of materialism and conceptions of the body as machine; nineteenth-century automata provided writers and artists with a way of negotiating conflicts between individual desires and social constraints; other automata embodied an industrializing machine age and its new technologies; fin-de-siècle androids manifested a modern privileging of logic and system over individual volition or free will; still others were formed out of eugenicist dreams of human perfection. Of course, there are many other possibilities here, for automata have had as many uses as they have had forms. For all their variety though, they invariably appear at the intersection of science and the arts: from René Descartes's seventeenthcentury musings on clockwork humans and 'beast-machines' to the eighteenth-century materialist Julien Offray de La Mettrie's deliberations on Vaucanson's famous artificial duck; from the master of macabre Edgar Allan Poe's writing about Kempelen's celebrated chess-playing 'Turk' to the American inventor Thomas Edison's nursery rhyme uttering dolls; and from the Maschinenmensch of Fritz Lang's Metropolis to the suburban gynoid of The Stepford Wives. These automata have also inspired influential theoretical work, from Freud's essay on 'The Uncanny ' (1919) to Donna Haraway's Simians, Cyborgs, and Women (1991), as well as a considerable body of literary and cultural history.
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