No abstract

HER2 TMD mutations represent rare but distinct targetable driver mutations in lung adenocarcinoma. CGP capable of detecting diverse HER2 alterations, including HER2 TMD mutations, should be broadly adopted to identify all patients who may benefit from HER2-targeted therapies.

Abscopal effect is a rare phenomenon characterized by tumor regression of untreated metastatic lesions after a local therapy (eg, radiotherapy). We studied the probability of abscopal effect with radiotherapy associated with anti-programmed death cell 1 (PD1) therapy after progression on anti-PD1. This study is a retrospective analysis of patients treated with nivolumab or pembrolizumab for melanoma, non-small cell lung cancer (NSCLC) and renal cancer at Antônio Ermírio de Moraes Oncology Center, Brazil. To be eligible for this analysis, patients must have had unequivocal evidence of disease progression on anti-PD1 therapy and subsequent radiotherapy for any tumor site while still receiving anti-PD1. The abscopal effect was characterized as a response outside the irradiated field after radiotherapy plus anti-PD1. Sixteen patients were evaluated, including 12 metastatic melanoma, 2 metastatic NSCLC, and 2 metastatic renal cell carcinoma. The median time to disease progression on anti-PD1 was 3 months. The radiotherapy field included lung, lymph nodes, and bones, with a median total dose of 24 Gy (1-40 Gy), usually in 3 fractions (1-10 fractions). Three patients with melanoma developed an abscopal effect at a rate of 18.7% (25% among melanoma patients). Of note, one of them achieved a remarkable complete response lasting >6 months. Three patients with melanoma obtained a significant local response after radiotherapy, despite no response in distant metastases. Eleven patients presented disease progression after radiotherapy. No increased toxicity was observed. In conclusion, no patients with NSCLC or renal cancer showed abscopal effect, but 25% of patients with melanoma showed regression of nonirradiated lesions when anti-PD1 was continued after radiation to a tumor site that had progressed on anti-PD1 monotherapy.

No abstract

We evaluated local opinion about reducing livestock losses to puma (Puma concolor) and the potential for conflict among livestock breeders inside a protected area in the highlands of a southeastern Brazilian Atlantic forest. We also quantified the number and type of livestock losses, and determined if predation by puma was correlated with property profile and landscape characteristics. We conducted semistructured interviews with 42 livestock breeders sampled in 36 rural properties. When asked how to reduce predation, 33% of livestock breeders refused to answer, 26% suggested improving livestock husbandry practices, 19% stated that there was no appropriate action, 17% favored removing the "problem" individual, and 5 % suggested killing the puma. Opinion on how to solve predation was independent of herd size and history of losses, and was correlated with respondent age class. Older respondents tended to suggest removing or killing pumas. Attitudes toward predation represented high potential for conflict among livestock breeders who demonstrated high discordance among responses. Horses were the most common prey (51%), followed by cattle (28%), sheep (17%), and goats (4%); totaling 47 animals attacked between 2004 and 2007. Annual predation was approximately 12 ± 5 animals, equivalent to 0.4% of the total livestock. Property elevation and distance from the urban center were the main predictors of predation probability. This survey used a novel approach that has not been addressed directly in other studies on livestock predation and demonstrated that the high potential for conflict among livestock breeders should be considered before implementing management actions.

A dose distribution map was generated based on the estimated doses received, which could allow prediction of the areas most affected by radiation and facilitate further correlations with dental complications after radiotherapy. © 2016 Wiley Periodicals, Inc. Head Neck 38: 1708-1716, 2016.

The composite Gel-Alanine phantom can be used for the end-to-end test of a single isocenter VMAT for simultaneous treatment of multiple brain metastases. The use of the alanine as the lesion cores for the treatment planning provided the precise dose measurements inside each lesion and allowed the conversion of the gel R2 values based on an accurate dose measurement inside the target.

Considerable resources have been allocated to support research in the development of noninvasive and non-destructive techniques for carcass composition and quality evaluation. Ultrasonography is a reliable and relatively low-cost technique that can be used. In the present study, real-time ultrasonography was used to predict ribeye area (REA) and subcutaneous fat thickness (FT) in live animals as compared to carcass measurements. Animals used were 115 yearling bull calves (initial body weight, 329 kg), kept under feedlot conditions, of four genetic groups (30, ½ Angus x Nellore; 30, ½ Canchim x Nellore; 30, ½ Simmental x Nellore, and 25 Nellore), and two finishing frame sizes (small and large). Four ultrasonographic measurements were taken every 28 days until slaughter. Predictive precision of ultrasonographic measurements increased as animals approached slaughter, reaching maximum values at the last measurement (R 2 =0.68 and 0.82 for REA and FT, respectively). FT carcass measurements was influenced by genetic group and live measurements (P < 0.05). Frame size did not influence REA and FT, probably due to small, but distinctive differences among genetic groups.