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The detailed structure of an 0-glycan derived from the fruit of L y c h bmbmum L. was elucidated based on glycosidic linkage analysis, complete and partial acid hydrolysis, 'H-NMR and u C N M R spectroscopy. According to the experiments, the carbohydrate was in the form of pdysacchride ( a r a b i i a c t a n ) chains with highly branched 3,4-gaktans and terminal arabiiwfumnasyl Substitwnts. and its glycan were investigated using tritium thymidine incorm b t y s h i f f assay ( M A ) . The results suggested that the irmnunoactive components of the fruit of Ly&m bmbmwn L. could enhance the splenocyte proliferation in n o d mice and the effects of glycan chain were stronger than those of glycoconjugate. The target cell was most likely to be B-lymphocyk, on which existed receptor binding site acting with the glycan. In addition, the inunmstimulabry effect of glycoconjugate (LbGp4) and its glycan (LbGPQ-OL) was d a ted with activating the expression of nuclear factor ICE (NFd) and activator protein 1 (m-1).The immun*modulating mechanrsn * of glycoooly'ugate poration assay, flow cytometry assay and eiectrophoretical

Keywords Lyciwn bar6onun 15. , glycoconjugate , activity * (~1 0 3 0 7 1 LI, L.T.; DONG, L.J.)

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