Studies on chemistry and immuno‐ modulating mechanism of a glycoconjugate from Lycium barbarum L.

Abstract

The detailed structure of an 0-glycan derived from the fruit of L y c h bmbmum L. was elucidated based on glycosidic linkage analysis, complete and partial acid hydrolysis, 'H-NMR and u C N M R spectroscopy. According to the experiments, the carbohydrate was in the form of pdysacchride ( a r a b i i a c t a n ) chains with highly branched 3,4-gaktans and terminal arabiiwfumnasyl Substitwnts. and its glycan were investigated using tritium thymidine incorm b t y s h i f f assay ( M A ) . The results suggested that the irmnunoactive components of the fruit of Ly&m bmbmwn L. could enhance the splenocyte proliferation in n o d mice and the effects of glycan chain were stronger than those of glycoconjugate. The target cell was most likely to be B-lymphocyk, on which existed receptor binding site acting with the glycan. In addition, the inunmstimulabry effect of glycoconjugate (LbGp4) and its glycan (LbGPQ-OL) was d a ted with activating the expression of nuclear factor ICE (NFd) and activator protein 1 (m-1).The immun*modulating mechanrsn * of glycoooly'ugate poration assay, flow cytometry assay and eiectrophoretical

Cell wall polysaccharides of Chinese Wolfberry (Lycium barbarum): Part 2. Characterisation of arabinogalactan-proteins

“…␣-l-Rhamnopyranosyl residues also existed as terminal sugars. The structural features reported here contradict earlier reports that Wolfberry AGPs carry a backbone of (1 → 4)-linked ␤-dgalactopyranosyl residues (Huang et al, 1999;Peng, Huang, et al, 2001) and confirm the assignment of Zhang and Zhang (2007) that the backbone is a (1 → 3)-␤-d-galactan.…”

“…Glycoconjugates in the form of arabinogalactanproteins (AGPs) were identified, confirming several previous reports of these molecules in Wolfberry fruit. In two of these publications (Huang, Tian, & Zheng, 1999;Peng, Huang, Qi, Zhang, & Tian, 2001) it was reported that the glycoconjugates LbGp3 and LbGp4 were AGPs containing a (1 → 4)-linked galactopyranan backbone frequently substituted at O-3 with highly branched side chains terminated predominantly by arabinofuranosyl residues. The authors pointed out the novelty of this structure, as most known plant-derived AGPs carry a (1→ 3)-linked galactopyranan backbone substituted at O-6 with side chains containing additional galactopyranosyl residues as well as arabinofuranosyl residues.…”

Antitumor activity of Lycium barbarum polysaccharides with different molecular weights: an in vitro and in vivo study

“…In fact, LBP is extracted from the edible Chinese herb, Lycium barbarum. Previous studies have shown that LBP could enhance the proliferation of splenocytes in mice [34,35], activate macrophages [36], and promote both the phenotypic and functional maturation of DC [37]. Besides, LBP also has various other biological activities, such as neuroprotective effects [38,39], radioprotective activity [40], cardioprotective activity [41], and hepatoprotective effects [42,43].…”

Goji-Saft, ein neuer Wundertrank für Langlebigkeit und Wohlbefinden? Eine Übersicht zu Inhaltsstoffen, Pharmakologie, Wirkversprechen und Nutzen

“…There are evidences that LBP act through enhanced expression of various cytokines and transcription factors. The protein polysaccharide complex LbGp4 stimulates the expression of nuclear factor κB (NfκB) and activator protein 1 (AP-1) [15]. LBP3 was shown to increase the expression of interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) at the mRNA and protein levels in cultures of human peripheral blood mononuclear cells [44].…”

“…LBP have been shown in several studies to promote splenocyte proliferation in vitro [14,15,43]. There are evidences that LBP act through enhanced expression of various cytokines and transcription factors.…”