Shoot meristems of plants are composed of stem cells that are continuously replenished through a classical feedback circuit involving the homeobox WUSCHEL (WUS) gene and the CLAVATA (CLV) gene signaling pathway. In CLV signaling, the CLV1 receptor complex is bound by CLV3, a secreted peptide modified with sugars. However, the pathway responsible for modifying CLV3 and its relevance for CLV signaling are unknown. Here we show that tomato inflorescence branching mutants with extra flower and fruit organs due to enlarged meristems are defective in arabinosyltransferase genes. The most extreme mutant is disrupted in a hydroxyproline O-arabinosyltransferase and can be rescued with arabinosylated CLV3. Weaker mutants are defective in arabinosyltransferases that extend arabinose chains, indicating that CLV3 must be fully arabinosylated to maintain meristem size. Finally, we show that a mutation in CLV3 increased fruit size during domestication. Our findings uncover a new layer of complexity in the control of plant stem cell proliferation.
The authors report on the performance of an Yb-doped, 100μm core rodlike photonic crystal fiber (PCF) used as the final amplifier in a gain-staged master-oscillator/power-amplifier source. From the PCF, they obtained 1-ns-long pulses of energy in excess of 4.3mJ, peak/average power ∼4.5MW∕42W, and spectral linewidth ∼20GHz. The PCF emitted a beam exhibiting near-Gaussian, single-transverse-mode profile of M2∼1.3.
We report on a diode-pumped master-oscillator/fiber-amplifier (MOFA) system consisting of a passively Q-switched, single-frequency Nd:LSB microchip laser that seeds a dual-stage Yb-doped fiber amplifier. A large-core, single-mode photonic crystal fiber was used for the final amplifier. The MOFA generated 1062nm wavelength, 1-ns long, ~10kHz repetition-rate, diffraction-limited pulses of energy >1mJ, peak power >1MW, average power >10W, and spectral linewidth ~9 GHz.
Abstract. We demonstrate a record bandwidth high energy supercontinuum source suitable for multispectral photoacoustic microscopy. The source has more than 150 nJ∕10 nm bandwidth over a spectral range of 500 to 1600 nm. This performance is achieved using a carefully designed fiber taper with large-core input for improved power handling and small-core output that provides the desired spectral range of the supercontinuum source.
A Q-switched microchip laser generating 1064 nm wavelength, subnanosecond pulses at a 13.4 kHz repetition rate was used to seed a dual-stage amplifier featuring a 40 microm core Yb-doped photonic-crystal fiber (PCF) as the power amplifier. From this source, we obtained diffraction-limited (M2 = 1.05), approximately 450 ps pulses of energy > 0.7 mJ, peak power in excess of 1.5 MW, and an average power of approximately 9.5 W. By further amplifying the PCF output in a multimode 140 microm core Yb-doped fiber, we generated a peak power in excess of 4.5 MW, the highest obtained in a fiber source to our knowledge.
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