Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

Brooks, Christopher D.; Nekrasov, Vladimir; Lippman, Zachary B.; Eck, Joyce Van

doi:10.1104/pp.114.247577

Cited by 630 publications

(435 citation statements)

References 28 publications

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“…Highly efficient, stable mutagenesis was subsequently demonstrated in Arabidopsis, as well as in several crops: rice, wheat, barley, Brassica oleracea , potato, maize and soybean (Brooks et al ., 2014; Butler et al ., 2015; Čermák et al ., 2015; Fauser et al ., 2014; Feng et al ., 2014; Ito et al ., 2015; Jiang et al ., 2014; Lawrenson et al ., 2015; Li et al ., 2015; Svitashev et al ., 2015; Wang et al ., 2014; Zhang et al ., 2014; Zhou et al ., 2014). The CRISPR‐Cas9 system can be used to achieve multiple mutagenesis by the expression of several sgRNAs targetting different genes (Lowder et al ., 2015; Ma et al ., 2015).…”

Section: Introductionmentioning

confidence: 99%

Selective gene dosage by CRISPR‐Cas9 genome editing in hexaploid Camelina sativa

Morineau

Bellec

Tellier

et al. 2017

Plant Biotechnology Journal

225

151

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In many plant species, gene dosage is an important cause of phenotype variation. Engineering gene dosage, particularly in polyploid genomes, would provide an efficient tool for plant breeding. The hexaploid oilseed crop Camelina sativa, which has three closely related expressed subgenomes, is an ideal species for investigation of the possibility of creating a large collection of combinatorial mutants. Selective, targeted mutagenesis of the three delta‐12‐desaturase (FAD2) genes was achieved by CRISPR‐Cas9 gene editing, leading to reduced levels of polyunsaturated fatty acids and increased accumulation of oleic acid in the oil. Analysis of mutations over four generations demonstrated the presence of a large variety of heritable mutations in the three isologous CsFAD2 genes. The different combinations of single, double and triple mutants in the T3 generation were isolated, and the complete loss‐of‐function mutants revealed the importance of delta‐12‐desaturation for Camelina development. Combinatorial association of different alleles for the three FAD2 loci provided a large diversity of Camelina lines with various lipid profiles, ranging from 10% to 62% oleic acid accumulation in the oil. The different allelic combinations allowed an unbiased analysis of gene dosage and function in this hexaploid species, but also provided a unique source of genetic variability for plant breeding.

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Section: Introductionmentioning

confidence: 99%

Selective gene dosage by CRISPR‐Cas9 genome editing in hexaploid Camelina sativa

Morineau

Bellec

Tellier

et al. 2017

Plant Biotechnology Journal

225

151

View full text Add to dashboard Cite

show abstract

“…Since 2013, CRISPR/Cas9 has been successfully applied by transient expression and/or stable transgenic lines in several plant species, such as Arabidopsis, Nicotiana benthamiana, rice, wheat, maize, and tomato (Brooks et al 2014;Jiang et al 2013;Li et al 2013;Miao et al 2013;Nekrasov et al 2013;Shan et al 2013). Moreover, the mutations generated in the primary transgenic plants by the CRISPR/Cas9 system can be stably transmitted to the next generation (Brooks et al 2014;Feng et al 2014).…”

mentioning

confidence: 99%

“…Moreover, the mutations generated in the primary transgenic plants by the CRISPR/Cas9 system can be stably transmitted to the next generation (Brooks et al 2014;Feng et al 2014). Thus, the CRISPR/Cas9 system is becoming a powerful tool for genome editing in plants, whereas the reports of the usage and efficiency of the CRISPR/Cas9 system-mediated plant genome engineering are still limited.…”

mentioning

confidence: 99%

Efficient targeted mutagenesis in potato by the CRISPR/Cas9 system

et al. 2015

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“…Other than model plants, genome editing with the CRISPR/Cas9 system has been performed in many crop plants, e.g., maize [57], rice [54,58], sorghum [59], soybean [60,61], tomato [62], and wheat [54,63]. These reports demonstrated (1) a high-frequency of mutation following gene transfer of sgRNA/Cas9 via Agrobacterium-mediated transformation, with a variety of mutation rates depending on species; in rice, mutation efficiency reached 100 % [58], (2) the achievement of biallelic mutations after gene transfer of sgRNA/Cas9 in the T0 generation in several crop plants [58,[60][61][62], and (3) that multiplex gRNA expression can lead to simultaneous mutation of multiple target genes [54,58,[60][61][62][63]. Biallelic mutations produced by the introduction of gRNA/ Cas9 genes will have the advantage of obtaining plants with a knockout phenotype at the T0 generation, which will reduce the breeding period.…”

Section: Molecular Breeding: Genomics and Genetic Engineering In Woodmentioning

confidence: 99%

Genome engineering of woody plants: past, present and future

2016

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Engineered endonucleases that digest the specific sequences can be used to modify target genomes precisely. This is called ''genome editing'' and is used widely to modify the genome of various organisms. The DNA-binding domains of zinc finger (ZF) proteins were the first to be used as a genome editing tool, in the form of designed ZF nucleases and, more recently, transcription activator-like effector as well as the clustered, regularly interspaced, short palindromic repeats and CRISPR-associated protein 9 (CRISPR/Cas9) system that targets RNA-DNA rather than protein-DNA interactions have been used successfully. These are powerful tools with which targeted gene modifications can be introduced in various organisms, including various plant species. A key step in genome editing is the generation of a double-stranded DNA break that is specific to the target gene. This is achieved using custom-designed endonucleases, which enable site-directed mutagenesis via a non-homologous end joining repair pathway and/or gene targeting via homologous recombination to occur efficiently at specific sites in the genome. This review provides an overview of the current status of genomics and genetic engineering of woody plants, and recent advances in genome editing technologies in plants as well as fungi. We also discuss how these strategies can provide insights into molecular breeding technology for woody plants.

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Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

Cited by 630 publications

References 28 publications

Selective gene dosage by CRISPR‐Cas9 genome editing in hexaploid Camelina sativa

Selective gene dosage by CRISPR‐Cas9 genome editing in hexaploid Camelina sativa

Efficient targeted mutagenesis in potato by the CRISPR/Cas9 system

Genome engineering of woody plants: past, present and future

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