The characteristics of Cs accumulation and localization in edible mushrooms were examined using the mycelia of Pleurotus ostreatus-Y1. Scanning electron microscope images revealed the existence of white spots, and energy dispersive X-ray microanalyzer analysis indicated the presence of larger amounts of Cs and P in these spots in mycelia cultured on medium containing 25 mM CsCl. The (137)Cs activities in the mycelia were approximately 4-6 times higher than those in water used for (137)Cs elution. Higher Cs concentrations in the sediment fraction including vacuolar pellets were obtained compared to the upper fractions. It was observed that yellowish spots caused by the fluorescence of 4',6-diamidino-2-phenylindole (DAPI)-stained polyphosphate were localized in the mycelia. The higher fluorescence intensity of the yellowish-grained spots was measured in comparison with other regions in the mycelium. These results suggested that Cs in the mycelia was trapped by polyphosphate in vacuoles or other organelles.
RESUMO-O objetivo deste trabalho foi avaliar o efeito do uso combinado da lipase de Burkholderia cepacia (LBC) com a de Thermomyces lanuginosus (LTL) na produção de ésteres etílicos do óleo da polpa da macaúba. Os resultados obtidos por meio das enzimas livres foram comparados àqueles observados paras as enzimas imobilizadas. Em ambos os casos, observou-se a sinergia entre os biocatalisadores. O procedimento de imobilização influenciou o desempenho biocatalítico: 77,7 ± 3,2% de rendimento em ésteres foram obtidos com o par de lipases imobilizadas e 67,9±1,3% com o par de livres.
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