Description of the subject. Cashew was introduced to Côte d'Ivoire in 1951 to control erosion and reforest cutover lands. From 1972 to 1980, natural forest plantations were converted to fruit orchards and were supplemented by the ‘Jumbo’ cashew variety imported from Brazil. Germplasm expeditions conducted in 2010 and 2014–2015 identified 209 high-yielding cultivars in the major cashew growing areas of Côte d’Ivoire. Although the morphological characteristics of these cultivars have been assessed, little is known about the genetic diversity and genetic structure of the germplasm collection. Objectives. The objective of the study was to evaluate the genetic diversity of high-yielding cashew cultivars for better use in breeding programs. Method. We performed DNA isolation using Qiagen DNeasy Plant Mini Kits and PCR analysis with 18 SSR markers. Results. We identified the first two introduced populations of cashew in Côte d’Ivoire. The average allelic richness is 3.56 (± 1.45) alleles per locus, the fixation index (FIS) indicates an overall heterozygosity deficit of 0.332 (± 0.076), and the average population differentiation (FST) is 0.014 (± 0.004). Much of the total genetic variability occurs at the intra-population level (98.6%), compared to only 1.4% variability attributable to differences between populations. Average value of gene flow is 22.528. Conclusions. Gene flow within cashew populations maintains high intra-population genetic diversity. This flow rate reflects a long-term exploitable genetic variability for use in selection and conservation.

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The parasite weed Alectra vogelii (Benth) causes significant yield reduction of cowpea in Africa. To find and map the resistance gene to A. vogelii in cowpea, a F 2 population from a cross involving a resistant parent IT81D-994 and a susceptible TVX3236 was screened. Amplified fragment length polymorphism (AFLP) in combination with Single Sequence Repeat (SSR) analysis was used to identify markers that may be linked to the gene Rav 3 conferring resistance to A. vogelii in the cowpea cultivar IT81D-994. The analysis of F 2 individuals scored for resistance showed that a single dominant gene conditioned A. vogelii resistance in IT81D-994 with a probability of 30 to 50%. Sixty-six AFLP/SSR markers were identified. Using MAPMAKER, four linkage groups were found. The first group showed 33 markers linked to the susceptible gene. The closest identified marker was 21.4 centimorgans away from the susceptible gene. The primer combination that showed the closest linkage was 809ACG8 (E-ACG 8 + UBC-809). The second group indicated 4 markers linked together while the third and the fourth groups showed 2 markers linked together respectively. No markers were found linked to the resistance gene Rav 3 conferring the resistance in the cultivated cowpea cultivar IT81D-994.

Garcinia kola Heckel, called "petit cola" in Côte d'Ivoire and "bitter kola" in Nigeria, is a Non-Timber Forest Product of great socioeconomic importance. Unfortunately, this species is threatened due to overexploitation and deforestation. This study tested the effect of leaf area on stem cuttings to regenerate vegetatively in a non-mist poly-propagator without using exogenous hormones. Three stem cuttings types: greenwood, softwood and hardwood (12 -15 cm in length) were collected from 24-month years old seedlings. The two top leaves of each cutting type were conserved at different leaf area: 0 cm 2 , 28.25 cm 2 , 56.5 cm 2 , 84.75 cm 2 and 113 cm 2 (entire leaf area). Observations were made on rooting, sprouting and leafing abilities of cuttings depending to treatments applied. Results showed that all cutting types have presented good ability to shooting and rooting. Cuttings with 113 cm 2 leaf areas presented the best rooting (94.44% ± 2.42%) and shouting (95.55% ± 2.42%) percentage and longest primary root length (12.03 ± 0.50 cm). Hardwood, softwood and greenwood cuttings with 113 cm 2 leaf area had the best rooting percentage (96.66% ± 3.33%, 96.66% ± 5.77% and 90.00% ± 5.77% respectively). The longest primary root length (13.50 ± 0.97 cm) was observed to greenwood cuttings with 113 cm 2 leaf area. Regenerated plants in the forest presented well survival percentage (96.66%). This study shows that it is possible to regenerate entire plants by cuttings without using

Abstract:Mango is the third largest export fruit in Côte d'Ivoire. However, its yield is limited by the damage caused by fruit flies (Diptera: Tephritidae). This study aimed at assessing the trapping system for fruit fly detection and monitoring in mango orchards in Northern Côte d'Ivoire. Four sites were selected in Northern Côte d'Ivoire in mango production area and in timber yard areas. Five trapping systems were tested: two food attractants (torula yeast, CeraTrap) and three sex attractants (methyl eugenol, trimedlure, cue-lure). Plastic bottles were used for food attractants and commercial traps for sex attractants. Five traps per treatment with three repetitions per site were set up. Ten species of flies have been identified: Bactrocera dorsalis, B. cucurbitae, Ceratitis anonae, C. bremii, C. capitata, C. cosyra, C. punctata, Dacus ciliatus, D. punctatifrons and Dacus spp.. Among these species, B. dorsalis was dominant. It was captured by 94.04% and 90.94%, respectively, in mango orchards and forest areas included wild fruits. The average number of individuals captured varied from one species to another, depending on the attractants. This average was estimated to 379.91, 27.58, 25.55, 15.1 and 11.93, respectively, for méthyl eugénol, torula yeast, CeraTrap, cue-lure, trimedlure. The attractants have shown their specificity in capturing fly species. Methyl eugenol had the highest capture and was the most effective.