Histone deacetylase (HDAC) inhibitors can induce various transformed cells to undergo growth arrest and/or death. Suberoylanilide hydroxamic acid (SAHA) is an HDAC inhibitor which is in phase I/II clinical trials and has shown antitumor activity in hematologic and solid tumors at doses well tolerated by patients. HDAC is the target for SAHA, but the mechanisms of the consequent induced death of transformed cells are not completely understood. In this study, we report that SAHA induced polyploidy in human colon cancer cell line HCT116 and human breast cancer cell lines, MCF-7, MDA-MB-231, and MBA-MD-468, but not in normal human embryonic fibroblast SW-38 and normal mouse embryonic fibroblasts. The polyploid cells lost the capacity for proliferation and committed to senescence. The induction of polyploidy was more marked in HCT116 p21 WAF1 À/À or HCT116 p53À/À cells than in wild-type HCT116. The development of senescence of SAHA-induced polyploidy cells was similar in all colon cell lines. The present findings indicate that the HDAC inhibitor could exert antitumor effects by inducing polyploidy, and this effect is more marked in transformed cells with nonfunctioning p21 WAF1 or p53 genes. (Cancer Res 2005; 65(17): 7832-9)
By using electrochemical activated FTO glass as both a substrate and catalyst, we developed a facile and fast method to fabricate a WO 3 $2H 2 O thin film with a thickness of $100 nm. Due to the specific layered crystal structure of the dihydrated phase and the good contact with the transparent conductive oxides (TCOs) layer, the as-prepared WO 3 $2H 2 O thin film achieved a fast coloration/bleaching response speed (t c,90% ¼ 3.2 s, t b,90% ¼ 1.2 s), excellent cyclic stability, wide optical modulation range up to 53.8% and a high coloration efficiency of 107.8 cm 2 C À1 . A solid-state electrochromic device with a size of 5 cm  5 cm was also fabricated, exhibiting high transparency contrast and good reversibility between the bleached and colored states.
On demand routing protocols provide scalable and cost-effective solutions for packet routing in mobile wireless ad hoc networks. The paths generated by these protocols may deviate far from the optimal because of the lack of knowledge about the global topology and the mobility of nodes. Routing optimality affects network performance and energy consumption, especially when the load is high. In this paper, we define routing optimality using different metrics such as path length, energy consumption along the path, and energy aware load balancing among the nodes. We then propose a framework of Self-Healing and Optimizing Routing Techniques (SHORT) for mobile ad hoc networks. While using SHORT, all the neighboring nodes monitor the route and try to optimize it if and when a better local subpath is available. Thus SHORT enhances performance in terms of bandwidth and latency without incurring any significant additional cost. In addition, SHORT can be also used to determine paths that result in low energy consumption or to optimize the residual battery power. Thus, we have detailed two broad classes of SHORT algorithms: Path-Aware SHORT and Energy-Aware SHORT. Finally, we evaluate SHORT using the ns-2 simulator. The results demonstrate that the performance of existing routing schemes can be significantly improved using the proposed SHORT algorithms.
On stably replicating episomes, transcriptional activation of the -globin promoter by the -globin locus control region HS2 enhancer is correlated with an increase in nuclease sensitivity which is limited to the TATAproximal nucleosome (N1). To elucidate what underlies this increase in nuclease sensitivity and the link between chromatin modification and gene expression, we examined the nucleoprotein composition and histone acetylation status of transcriptionally active and inactive promoters. Micrococcal nuclease digestion of active promoters in nuclei released few nucleosome-like nucleoprotein complexes containing N1 sequences in comparison to results with inactive promoters. We also observed that N1 DNA fragments from active promoters are of a subnucleosomal length. Nevertheless, chromatin immunoprecipitation experiments indicate that histones H3 and H4 are present on N1 sequences from active promoters, with H3 being dramatically hyperacetylated compared with that from inactive promoters and vector sequences. Strikingly, H3 in the adjacent upstream nucleosome (N2) does not appear to be differentially acetylated in active and inactive promoters, indicating that the nucleosome modification of the promoter that accompanies transactivation by HS2 is highly directed and specific. However, global acetylation of histones in vivo by trichostatin A did not activate transcription in the absence of HS2, suggesting that HS2 contributes additional activities necessary for transactivation. N1 sequences from active promoters also contain reduced levels of linker histone H1. The detection of a protected subnucleosomal sized N1 DNA fragment and the recovery of N1 DNA sequences in immunoprecipitations using antiacetylated H3 and H4 antibodies argue that N1 is present, but in an altered conformation, in the active promoters.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.