Analytical method development and validation for the analysis therapeutic components and associated substances play an important role in the discovery, development and manufacture of pharmaceuticals and natural medicinal compounds. Pharmaceutical and herbal products formulated with more than one drug, typically referred to as combination products, are intended to meet previously unmet patients need by combining the therapeutic effects of two or more drugs in a single dosage. The new chemical entities and combination products can present daunting challenges to the analytical chemist responsible for the development and validation of analytical methods. The official test methods that result from these processes are used by quality control laboratories to ensure the identity, purity, potency, and performance of drug products [1].Analytical method development and validation though often considered routine, too little attention is paid to them with regards for their potential to contribute to overall developmental time and cost efficiency. These method-related activities are interrelated. They are iterative, particularly during early drug development phases. Parts of each process may occur concurrently or be refined at various phases of drug development. Changes encountered during drug development may require modifications to existing analytical methods. These modifications may require additional validation or transfer activities. In this paper, the overview the
Aim:The tremendous chemical diversity of nature is an attractive source of myriad chemotherapeutic agents. This fact aided by the virtual revolution in tissue culture technology facilitates employment of high throughput, In-vitro cytotoxic assays using human derived cancer cell lines to discover meticulous drug molecules. The present study was initiated to evaluate the anti proliferative potential of whole plant ethanolic extract of Corallocarpus epigaeus against human chronic myeloid leukaemia K562 cell lines by Trypan blue and MTT assay. Method: A trend in the reduction of total viable cell count was evident following a 24 hr single exposure to different concentrations of test extract in Trypan blue as well as in MTT assay. Result: Thus the ethanolic extract of Corallocarpus epigaeus has appreciable growth inhibitory activity on K562 cell lines with an IC50 value of 78.84µg/ml.
Conclusion:Further studies on various extracts of Corallocarpus epigaeus can be initiated to explore its potential as a promising phytochemical in anti-cancer therapy.
Pamburus missionis Swingle[1] is a small thorny shurb commonly called as kattunaranthi in tamil belonging to the family Rutaceae. The leaf of this plant is used traditionally for the treatment of fistula, joint swellings, rheumatism, fractures and piles. The anatomical and chemical investigations on Pamburus genus are very limited. Pamburus missionis Swingle is distributed in southern India. Earlier investigation was made on root and stem bark of Pamburus missionis S. and reported that it contains imperatorin, coumarins, diterpenes, flavones and xanthotoxis, isopimpinellin, scopoletin and luvangetin [2, 3].
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