A yellow-pigmented, non-motile, Gram-stain-negative, rod-shaped bacterium, designated II4T was obtained from soil sampled at Seongnam, Gyeonggi-do, Republic of Korea. Cells were strictly aerobic, grew optimally at 20–28 °C and hydrolysed casein. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain II4T formed a lineage within the family Xanthomonadaceae and clustered as members of the genus Lysobacter . The closest members were Lysobacter terrae THG-A13T (97.88 % sequence similarity), Lysobacter niabensis GH34-4T (97.82 %), Lysobacter oryzae YC6269T (97.74%), Lysobacter yangpyeongensis GH19-3T (97.53 %) and Lysobacter enzymogenes ATCC 29487T (96.18 %). The principal respiratory quinone was Q-8 and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The predominant cellular fatty acids were summed feature 9 (C16 : 0 10-methyl and/or iso-C17 : 1 ω9c) and iso-C15 : 0 and iso-C16 : 0. The DNA G+C content was 68.2 mol%. The average nucleotide identity and in silico DNA–DNA hybridization relatedness values between strain II4T and its closely related genus members with possible full genome sequences were ≤79.6 and 23.7 %, respectively. Based on genomic, chemotaxonomic, phenotypic and phylogenetic data, strain II4T represents novel species in the genus Lysobacter , for which the name Lyobacter terrestris sp. nov. is proposed. The type strain is II4T (=KACC 21196T=NBRC 113956T).
Materials and Methods Isolation and CultivationThe novel strain CYS-02 T was isolated from soil located at Suwon, Gyeonggi-do, Republic of Korea (37º15'37'' N and 126º56'17.2'' E). A modified culture method by 6-well Transwell plates (Corning, USA) was used for isolation. The Transwell plates were incubated in a shaker at 120 ×g for 4 weeks at 28 o C. After 4 weeks, the culture was serially diluted, and 100 μl of each dilution was spread onto R2A agar plates. Colonies were individually restreaked on R2A agar plates until pure colonies were obtained. For long-term preservation, cultures were stored as suspensions in R2A broth supplemented with 20% (v/v) glycerol at -70°C. Isolation, maintenance, and preservation of strain CYS-02 T were performed following the methodology described by Dahal RH et al. [14]. A white-pigmented, non-motile, gram-negative, and rod-shaped bacterium, designated CYS-02 T , was isolated from soil sampled at Suwon, Gyeonggi-do, Republic of Korea. Cells were strictly aerobic, grew optimally at 20-28ºC and hydrolyzed Tween 40. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CYS-02 T formed a lineage within the family Comamonadaceae and clustered as members of the genus Variovorax. The closest members were Variovorax guangxiensis DSM 27352 T (98.6% sequence similarity), Variovorax paradoxus NBRC 15149 T (98.5%), and Variovorax gossypii JM-310 T (98.3%). The principal respiratory quinone was Q-8 and the major polar lipids contain phosphatidylethanolamine (PE), phosphatidylethanolamine (PG), and diphosphatidylglycerol (DPG). The predominant cellular fatty acids were C 16:0 , summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c) and summed feature 8 (C 18:1 ω7c and/or C 18:1 ω6c). The DNA GC content was 67.7 mol%. The ANI and dDDH values between strain CYS-02 T and the closest members in the genus Variovorax were ≤ 79.0 and 22.4%, respectively, and the AAI and POCP values between CYS-02 T and the other related species in the family Comamonadaceae were > 70% and > 50%, respectively. The genome of strain CYS-02 T showed a putative terpene biosynthetic cluster responsible for antioxidant activity which was supported by DPPH radical scavenging activity test. Based on genomic, phenotypic and chemotaxonomic analyses, strain CYS-02 T was classified into a novel species in the genus Variovorax, for which the name Variovorax terrae sp. nov., has been proposed. The type strain is CYS-02 T (= KACC 22656 T = NBRC 00115645 T ).
In the article titled "Variovorax terrae sp. nov. Isolated from Soil with Potential Antioxidant Activity, the authors noticed the deposit number of NBRC was wrong, and thus are changing NBRC 00115645 to NBRC 115645 in two places within the paper.
A white-pigmented, non-motile, Gram-stain-negative, rod-shaped bacterium, designated CYS-01T, was obtained from soil sampled at Suwon, Gyeonggi-do, Republic of Korea. Cells were strictly aerobic, grew optimally at 28 °C. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain CYS-01T formed a lineage within the family Sphingobacteriaceae and clustered with members of the genus Pedobacter . The closest relatives were Pedobacter xixiisoli CGMCC 1.12803T (95.70 % sequence similarity), Pedobacter ureilyticus THG-T11T (95.35 %), Pedobacter helvus P-25T (95.28 %), Pedobacter chitinilyticus CM134L-2T (94.94 %), Pedobacter nanyangensis Q-4T (94.73 %) and Pedobacter zeaxanthinifaciens TDMA-5T (94.07 %). The principal respiratory quinone was MK-7 and the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid, unidentified lipids and an unidentified glycolipid. The predominant cellular fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and iso-C17 : 0 3-OH. The DNA G+C content was 36.6 mol%. Based on the results of genomic, chemotaxonomic, phenotypic and phylogenetic analyses, strain CYS-01T represents novel species in the genus Pedobacter , for which the name Pedobacter montanisoli sp. nov. is proposed. The type strain is CYS-01T (=KACC 22655T=NBRC 115630T).
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