Pedobacter montanisoli sp. nov., isolated from soil

Abstract

A white-pigmented, non-motile, Gram-stain-negative, rod-shaped bacterium, designated CYS-01T, was obtained from soil sampled at Suwon, Gyeonggi-do, Republic of Korea. Cells were strictly aerobic, grew optimally at 28 °C. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain CYS-01T formed a lineage within the family Sphingobacteriaceae and clustered with members of the genus Pedobacter . The closest relatives were Pedobacter xixiisoli CGMCC 1.12803T (95.70 % sequence similarity), Pedobacter ureilyticus THG-T11T (95.35 %), Pedobacter helvus P-25T (95.28 %), Pedobacter chitinilyticus CM134L-2T (94.94 %), Pedobacter nanyangensis Q-4T (94.73 %) and Pedobacter zeaxanthinifaciens TDMA-5T (94.07 %). The principal respiratory quinone was MK-7 and the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid, unidentified lipids and an unidentified glycolipid. The predominant cellular fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and iso-C17 : 0 3-OH. The DNA G+C content was 36.6 mol%. Based on the results of genomic, chemotaxonomic, phenotypic and phylogenetic analyses, strain CYS-01T represents novel species in the genus Pedobacter , for which the name Pedobacter montanisoli sp. nov. is proposed. The type strain is CYS-01T (=KACC 22655T=NBRC 115630T).

Pedobacter faecalis sp. nov., isolated from the faeces of eland, Taurotragus oryx

“…The quinones were eluted using a mobile phase comprising a methanol-isopropyl ether solution (3 : 1, v/v) at a flow rate of 0.7 ml min −1 [30]. Menaquinone-7 (MK-7) was identified as the major respiratory quinone of ELA7 T , which is typical of the genus Pedobacter [2][3][4]. Strain ELA7 T and the reference type species (P. ginsengisoli Gsoil 104 T and P. frigoris RP-3-15 T ) were cultured under identical conditions (22 °C for 24 h) to examine the fatty acid methyl ester profiles during exponential growth.…”

“…The genus Pedobacter is a Gram-stain-negative bacteria classified under the family Sphingobacteriaceae within the phylum Bacteroidota. Members of the genus Pedobacter exhibit aerobic characteristics, positive catalase and oxidase production, and display diverse colony colours such as white, yellow, orange, and pink [2][3][4][5][6]. At the time of writing, the genus Pedobacter consists of 110 species with validly published names, as documented in the List of Prokaryotic names with Standing in Nomenclature (LPSN) taxonomy browser database (https://lpsn.dsmz.de/genus/pedobacter).…”