From our data we can conclude that cryopreservation of spermatozoa from men with poor sperm quality does not negatively affect fertilization and pregnancy rates after ICSI. A larger study will be needed to investigate whether the use of cryopreserved spermatozoa can be helpful in selecting the most vital spermatozoa for ICSI.
We report the case of a 32-year-old woman suffering from severe liver dysfunction in the course of ovarian hyperstimulation syndrome (OHSS). Complications occurred after successful fertilization subsequent to ovarian stimulation with human menopausal gonadotropin followed by ovulation induction with human chorionic gonadotropin. Because of nausea, vomiting, abdominal distention and enlarged ovaries on an ultrasound examination, she was admitted on the diagnosis of OHSS. During the course of hospitalization severe hepatic injury developed. An increase of more than 100-fold in blood aminotransferase activity was observed. Applied treatment resulted in gradual reduction of ovarian size and resolution of ascites, as well as pleural and pericardial effusions. The patient was discharged from hospital after 46 days. Follow-up examinations at the 13th and 32nd weeks of gestation did not reveal any abnormalities. Pregnancy developed without complications and the woman went into spontaneous labor, giving birth to a viable child at 38 weeks' gestation. Taking into account the above case and previously published reports, the issue of liver dysfunction may have a great impact on the understanding both the pathology and the treatment of OHSS.
Introduction: Controlled ovarian hyperstimulation is an integral part of infertility treatment. Despite many years of use, some aspects of controlled ovarian stimulation have not yet been clarified, especially the role of the functional status of the ovaries before hormonal stimulation. Aim of the research: To assess the effect of the functional status of the ovaries on the embryological results of controlled ovarian hyperstimulation. Material and methods: The retrospective study included female patients treated for infertility. The patients were divided into two groups depending on the ultrasonographic appearance of the ovaries before controlled ovarian hyperstimulation. Patients with small antral follicles < 6 mm in diameter were selected for group I. Patients with five or more antral follicles ≥ 8 mm in diameter in each ovary were included in group II. Patients from both groups underwent the same treatment process. We performed a detailed analysis of the number, type and quality of the obtained embryos.
Results:The number of two-and three-blastomere embryos were comparable in the two groups. There were significantly more four-blastomere embryos in group I than II (p > 0.05). The numbers of A, C, D quality embryos were comparable between the groups (p > 0.05). There were more B quality embryos in group I than II (p > 0.05). The embryo growth rate was significantly faster in group I than II.
Conclusions:The results of the present study indicate that the functional status of the ovaries before controlled ovarian hyperstimulation plays a pivotal role in treatment outcome.
Purpose The present study was designed to investigate the impact of pressure on nuclear DNA integrity in viable cells of mouse blastocysts. Methods The blastocysts of hybrid F1 females [(C57Bl/10 J × CBA-H);N=15] aged 2-3 months were exposed into the pressure impulse lasting~0.021 s and characterized by a positive pressure peak of~76 mmHg. The nuclear DNA fragmentation index of mouse blastocysts was assessed by TUNEL assay within 60 s after exposure to pressure impulse. Results The mean nuclear DNA fragmentation index was significantly higher in the experimental group (83%) than in the control group (19.7%); p<0.001.
Conclusion(s)A low magnitude pressure impulse can induce nuclear DNA fragmentation in mouse blastocysts. The compression and decompression forces appearing during pressure fluctuations are responsible for the observed DNA shearing.
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