Influence of embryo transfer on blastocyst viability

Grygoruk, Cezary; Sieczyński, Piotr; Modliński, Jacek A.; Gajda, B.; Gręda, Paweł; Grad, I.; Pietrewicz, Piotr; Mrugacz, Grzegorz

doi:10.1016/j.fertnstert.2010.04.073

Cited by 11 publications

(15 citation statements)

References 8 publications

(8 reference statements)

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“…In the present study, much lower pressure changes,~76 mmHg, were able to cause pressure DNA shearing (PDS) inside the nucleus of a living cells. According to our recent studies, the pressure fluctuations of comparable magnitude could also appear during embryo transfer procedure (ET) and cause both morphological and apoptotic changes in the embryos exposed to ET [12,18]. Taking under consideration the entire process of extracorporeal fertilization, the significant pressure fluctuations could also emerge during oocyte pickup, oocyte decoronization and embryo pipetting.…”

Section: Discussionmentioning

confidence: 99%

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Pressure induced nucleus DNA fragmentation

Grygoruk¹,

Sieczyński

Modliński

et al. 2011

J Assist Reprod Genet

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Purpose The present study was designed to investigate the impact of pressure on nuclear DNA integrity in viable cells of mouse blastocysts. Methods The blastocysts of hybrid F1 females [(C57Bl/10 J × CBA-H);N=15] aged 2-3 months were exposed into the pressure impulse lasting~0.021 s and characterized by a positive pressure peak of~76 mmHg. The nuclear DNA fragmentation index of mouse blastocysts was assessed by TUNEL assay within 60 s after exposure to pressure impulse. Results The mean nuclear DNA fragmentation index was significantly higher in the experimental group (83%) than in the control group (19.7%); p<0.001. Conclusion(s)A low magnitude pressure impulse can induce nuclear DNA fragmentation in mouse blastocysts. The compression and decompression forces appearing during pressure fluctuations are responsible for the observed DNA shearing.

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Section: Discussionmentioning

confidence: 99%

“…For example, in the case of ET the pressure build up in the transferred liquid is proportional to the speed of ejection of the transferred load. For that reason, it is reasonable to suggest transferring the embryos at the lowest possible ejection speed [12,18].…”

Section: Discussionmentioning

confidence: 99%

Pressure induced nucleus DNA fragmentation

Grygoruk¹,

Sieczyński

Modliński

et al. 2011

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

show abstract

“…With high enough injection speeds, the shear stress can be strong enough to injure the vital cell's organs and impair embryo viability. A recent study indicated that fast ET, with an injection speed of the transferred volume of more than 1 m/sec, could trigger both morphological and apoptotic changes in mouse blastocysts (4,8). Alternatively, reduction of the injection speed to less than 0.1 m/sec allowed to avoid morphological changes and significantly decreased apoptosis in embryos (4).…”

Section: Discussionmentioning

confidence: 99%

“…Despite the variety of presently available catheters, the basic idea of delivering an embryo into the uterus remains the same: the pressure generated in the working chamber of the insulin syringe is passed into the catheter, where it causes the ejection of the transferred load. The study conducted previously by our team demonstrated that ET performed with the standard insulin syringe-catheter complex was able to affect mouse blastocyst viability (4). The abrupt alterations in the embryo's environment during ET were the most probable cause for the observed morphological and apoptotic changes in the mouse blastocysts (4,5).…”

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confidence: 84%