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Ethanol increased the frequency of miniature glycinergic currents [miniature inhibitory postsynaptic currents (mIPSCs)] in cultured spinal neurons. This effect was dependent on intracellular calcium augmentation, since preincubation with BAPTA (an intracellular calcium chelator) or thapsigargin [a sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) pump inhibitor] significantly attenuated this effect. Similarly, U73122 (a phospholipase C inhibitor) or 2-aminoethoxydiphenyl borate [2-APB, an inositol 1,4,5-trisphosphate (IP₃) receptor (IP3R) inhibitor] reduced this effect. Block of ethanol action was also achieved after preincubation with Rp-cAMPS, inhibitor of the adenylate cyclase (AC)/PKA signaling pathway. These data suggest that there is a convergence at the level of IP₃R that accounts for presynaptic ethanol effects. At the postsynaptic level, ethanol increased the decay time constant of mIPSCs in a group of neurons (30 ± 10% above control, n = 13/26 cells). On the other hand, the currents activated by exogenously applied glycine were consistently potentiated (55 ± 10% above control, n = 11/12 cells), which suggests that ethanol modulates synaptic and nonsynaptic glycine receptors (GlyRs) in a different fashion. Supporting the role of G protein modulation on ethanol responses, we found that a nonhydrolyzable GTP analog [guanosine 5'-O-(3-thiotriphosphate) (GTPγS)] increased the decay time constant in ∼50% of the neurons (28 ± 12%, n = 11/19 cells) but potentiated the glycine-activated Cl(-) current in most of the neurons examined (83 ± 29%, n = 7/9 cells). In addition, confocal microscopy showed that α1-containing GlyRs colocalized with Gβ and Piccolo (a presynaptic cytomatrix protein) in ∼40% of synaptic receptor clusters, suggesting that colocalization of Gβγ and GlyRs might account for the difference in ethanol sensitivity at the postsynaptic level.

The oral health-related quality of life of children with a history of CL/P was similar to that of the control group. Nevertheless, a lower quality of life was observed concerning items associated with speech and being understood by other people. Further study into the risk factors associated with surgery and rehabilitative treatment is recommended.

BackgroundThe purpose of this study is to determine the effectiveness of warming anesthesia on the control of the pain produced during the administration of dental anesthesia injection and to analyze the role of Transient Receptor Potential Vanilloid-1 nociceptor channels in this effect.Patients and methodsA double-blind, split-mouth randomized clinical trial was designed. Seventy-two volunteer students (22.1±2.45 years old; 51 men) from the School of Dentistry at the Universidad Austral de Chile (Valdivia, Chile) participated. They were each administered 0.9 mL of lidocaine HCl 2% with epinephrine 1:100,000 (Alphacaine®) using two injections in the buccal vestibule at the level of the upper lateral incisor teeth. Anesthesia was administered in a hemiarch at 42°C (107.6°F) and after 1 week, anesthesia was administered by randomized sequence on the contralateral side at room temperature (21°C–69.8°F) at a standardized speed. The intensity of pain perceived during the injection was compared using a 100 mm visual analog scale (VAS; Wilcoxon test p<0.05).ResultsThe use of anesthesia at room temperature produced an average VAS for pain of 35.3±16.71 mm and anesthesia at 42°C produced VAS for pain of 15±14.67 mm (p<0.001).ConclusionThe use of anesthesia at 42°C significantly reduced the pain during the injection of anesthesia compared to its use at room temperature during maxillary injections. The physiological mechanism of the temperature on pain reduction could be due to a synergic action on the permeabilization of the Transient Receptor Potential Vanilloid-1 channels, allowing the passage of anesthetic inside the nociceptors.

SUMMARY:From a biomechanical standpoint, bone geometry and density are factors correlated to the bone resistance of the femur when supporting body weight, with geometric parameters like the diameter of the femoral head and neck, the length of the femoral neck, and the femoral neck angle as determinant factors in the incidence of hip fractures, which increase in frequency and seriousness in osteoporotic patients. In Chile, morphometric data that contributes to relating the anatomy of the proximal epiphysis of the femur as an associated factor in hip fractures does not exist; likewise, there are no anthropometric indexes that may contribute to the forensic sciences. The purpose of this study is to establish average measurements of the proximal epiphysis of the femur in the adult Chilean population. Descriptive Study. The proximal epiphyses of 81 dry adult femurs were analyzed (44 right and 37 left bones), measuring the following parameters: length of the femoral neck (LN), femoral neck angle (FNA), circumference of the femoral head (CH) and circumference of the femoral neck (CN). The statistical relationship between the measurements and the side of each sample was analyzed (t-test p=0.05). The average lengths were LN= 3.59cm (±0.43 cm); FNA= 124.17º (±6.37º), CH= 14.34 cm (±1.27 cm) and CN= 9.7 cm (±0.87 cm). No significant differences between the left and right sides were found. Average numbers were obtained for the anatomy of the proximal femoral epiphysis from a sample in the Chilean population. With the data obtained, we propose to carry out anatomoclinical, epidemiologic and forensic studies in this population.

Vertebrate skin contains specialized sensory organs collectively referred to as sensory corpuscles, which are supplied by peripheral processes of primary sensory neurons that encode non-painful mechanical stimuli (low-threshold mechanoreceptors, LTMRs; Abraira & Ginty, 2013; Fleming & Luo, 2013; Zimmerman et al., 2014). A group of these structures localized in the erogenous zones are involved in sexual pleasure. In women, the glans clitoris is generally considered the structure most involved in sexual pleasure, and it is a key element required to reach orgasm. It is a fibrovascular, non-erectile, densely innervated structure located in the midline that is the only

SUMMARY:Determining the volume of the maxillary sinus is necessary in certain procedures. We present a simple method for estimating the volume of the maxillary sinus from groups 3, 5 and 9 coronal CT scan using the Cavalieri method combined with systematic sampling and evaluated randomized images. We used 10 dry skulls submitted to the TC. The actual volume was determined using silicone adapted to the maxillary sinus cavity. The estimated volume showed a high correlation with real volume, with no differences between groups. We concluded that it is possible to estimate without bias and with high accuracy the volume of the maxillary sinus from a minimum of 3 CT images obtained through randomized systematic sampling and the proposed method.

This paper describes the design, planning, and successful installation of a fit-for-purpose casing patch to isolate a water producing zone, the subsequent perforation of an adjacent zone, and a gravel pack completion in the same well for the first time worldwide. The proximity of the zones and the sand control requirements made the design and planning of this job a challenging task that is detailed in this manuscript. The main producing zone in the SW-21 well watered out after few years of production. A second target was identified located just four feet below the main zone. To extend the life of the well and to add reserves from the secondary target, the upper 100% water zone had to be isolated. A fit-for-purpose, thin wall casing patch solution was designed to: allow perforation of the secondary target while maintaining patch integrity, allowing for the installation of sand control screens; and resist following gravel pack completion, by keeping the minimum recommended clearance between casing and screens, inside the minimal patch-reduced diameter. The re-completions program consisted of: 1. Successful recovery of existing gravel pack from the main producing zone and thorough wellbore cleanup. 2. Casing patch installation consisting of a 23-ft long patch to isolate the water-producing zone. In this case, a specific engineering design analysis was required to ensure that, because of the very close distances between zones, the patch would still maintain integrity during perforation of the secondary target and the resulting patch overlap. 3. Successful integrity test to confirm upper interval isolation before perforating the lower interval. 4. A precise perforating operation carried out to perforate the secondary zone. Based on engineering recommendations, some length of the installed patch was perforated to guarantee a minimum unperforated distance of casing patch between zones to guarantee patch sealing features. 5. Once perforation was successfully accomplished, a gravel pack completion—inside the casing patch reduced diameter—was executed along the new zone for sand control purposes, and the well was put into production. This paper presents the different interactions between a multidisciplinary research and development team, and completions and reservoir engineers to come up with a full solution for water isolation and sand control under such challenging conditions. For the first time in the world, a casing patch was used to isolate a water zone, and at the same time, perform a gravel pack completion inside the patch reduced diameter. Well performance, without any mechanical issues, confirms the success of the provided solution.