In this work, poly(HEMA-MAPA) membranes were prepared by UV-polymerization technique. These membranes were characterized by SEM, FTIR, and swelling studies. Synthesized membranes had high porous structure. These membranes were used for controlled release of curcumin which is already used as folk remedy and used as drug for some certain diseases and cancers. Curcumin release was investigated for various pHs and temperatures. Optimum drug release yield was found to be as 70% at pH 7.4 and 37 °C within 2 h period. Time-depended release of curcumin was also investigated and its slow release from the membrane demonstrated within 48 h.
In this study presented, p(HEMA) nanoparticles were synthesized by the emulsion polymerization technique and then activated by a silanization agent, 3-aminopropyltriethoxysilane (APTES). The APTES-functionalized p(HEMA) nanoparticles that were synthesized were characterized by studies using the Zetasizer, FTIR and SEM. The p(HEMA)-APTES nanoparticles were further modified with phenyl boronic acid (PBA), and these boronate affinity nanoparticles were used for the recognition of some sugars such as galactose, fructose and raffinose. The system parameters (temperature and initial sugar concentration) were optimized for maximum sugar adsorption. The maximum amount of galactose, fructose, and raffinose adsorbed were found to be 4334.5 mg/g; 4334.9 and 810.0 mg/g, respectively (at 25°C, in a phosphate buffer of pH 7.0). Considering the results of this study, it can be concluded that these nanoparticles may be used as a new alternative for the specific recognition of sugar.
The purification of immunoglobulin G (IgG) from human plasma was performed by using a novel metal-chelated adsorbent with nano size. The non-porous nanoparticles were produced by surfactant free emulsion polymerization of ethylene glycol dimethacrylate (EDMA) and 2-methacryloylamidohistidine (MAH). Then, Cu(II) ions were chelated on the nanoparticles. The nano-poly(EDMA-MAH) nanoparticles were characterized by Fourier transform infrared, scanning electron microscope, atomic force microscope and elemental analysis. The non-porous nanoparticles were spherical form and have 100-250 nm size distribution. The maximum IgG adsorption capacity of the Cu(II) chelated nanoparticles was found to be 463 mg/g polymer at pH 7.0 in HEPES buffer. Desorption of IgG was performed by 1.0 M NaCl and desorption rate was found to be 97 %. IgG was obtained from human plasma with purity of 94 % (up to 578 mg/g polymer). The non-porous nanoparticles allowed one-step purification of IgG from human plasma.
Non-porous poly(2-hydroxyethyl methacrylate) [p(HEMA)] nanoparticles were prepared by surfactant free emulsion polymerization. The p(HEMA) nanoparticles was about 200 nm diameter, spherical form, and non-porous. Reactive Red 120 (RR 120) was covalently attached to the p(HEMA) nanoparticles and Ni(II) ions were incorporated to attach dye molecules. Urease was immobilized onto RR120-Ni(II) attached p(HEMA) nanoparticles via adsorption. The maximum urease adsorption capacity of RR120-Ni(II) attached p(HEMA) nanoparticles was 480.01 mg g 21 nanoparticles at pH 7.0 in phosphate buffer. It was observed that urease could be repeatedly adsorbed and desorbed without significant loss in adsorption amount. K m values were 21.50 and 34.06 mM for the free and adsorbed enzyme. The V max values were 4 U for the free enzyme and 3.3 U for the adsorbed enzyme. The optimum pH was 25 mM pH 7 phosphate buffer for free and adsorbed enzyme. The optimum temperature was determined at 35 C and 55 C for the free and adsorbed enzyme, respectively. These findings show considerable promise for this material as an adsorption matrix in biotechnological applications.
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