Blister
fluid (BF) is a novel and viable research matrix for burn
injury study, which can reflect both systemic and local microenvironmental
responses. The protein abundance in BF from different burn severities
were initially observed using a 2D SDS-PAGE approach. Subsequently,
a quantitative data independent acquisition (DIA) method, SWATH, was
employed to characterize the proteome of pediatric burn blister fluid.
More than 600 proteins were quantitatively profiled in 87 BF samples
from different pediatric burn patients. These data were correlated
with clinically assessed burn depth and time until complete wound
re-epithelialization through several different statistical analyses.
Several proteins from these analyses exhibited significant abundance
change between different burn depth or re-epithelialization groups,
and can be considered as potential biomarker candidates. Further gene
ontology (GO) enrichment analysis of the significant proteins revealed
the most significant burn related biological processes (BP) that are
altered with burn depth, including homeostasis and oxygen transport.
However, for wounds with re-epithelialization times more or less than
21 days, the significant GO annotations were related to enzyme activity.
This quantitative proteomics investigation of burn BF may enable objective
classification of burn wound severity and assist with clinical decision-making.
Data are available via ProteomeXchange with identifier PXD011102.
The data presented here are associated with the article “The blister fluid proteome of paediatric burns” (Zang et al., 2016) [1]. Burn injury is a highly traumatic event for children. The degree of burn severity (superficial-, deep-, or full-thickness injury) often dictates the extent of later scar formation which may require long term surgical operation or skin grafting. The data were obtained by fractionating paediatric burn blister fluid samples, which were pooled according to burn depth and then analysed using data dependent acquisition LC–MS/MS. The data includes a table of all proteins identified, in which burn depth category they were found, the percentage sequence coverage for each protein and the number of high confidence peptide identifications for each protein. Further Gene Ontology enrichment analysis shows the significantly over-represented biological processes, molecular functions, and cellular components of the burn blister fluid proteome. In addition, tables include the proteins associated with the biological processes of “wound healing” and “response to stress” as examples of highly relevant processes that occur in burn wounds.
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