The blister fluid proteome of paediatric burns

Zang, Tuo; Broszczak, Daniel; Cuttle, Leila; Broadbent, James A.; Tanzer, Catherine; Parker, Tony J.

doi:10.1016/j.jprot.2016.06.026

Cited by 10 publications

(28 citation statements)

References 44 publications

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“…These strategies confirmed the presence of many of the above proteins in MF and added CCL-20/macrophage inflammatory protein-3b to the list for further investigation ( Table 2, complete proteome analysis reported in Supplemental Tables 1-5). Comparison with previously documented acute and chronic wound fluid proteomes (40,41) indicated that although MF exhibited several proteins in common with wound fluids (Tables 3 and 4), most were exclusive to MF (Supplemental Tables 1-5).…”

Section: Identification Of Proteins In Mf Vs Matched Pb Plasma: Protmentioning

confidence: 85%

Menstrual fluid factors facilitate tissue repair: identification and functional action in endometrial and skin repair

et al. 2018

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Repair after damage is essential for tissue homeostasis. Postmenstrual endometrial repair is a cyclical manifestation of rapid, scar-free, tissue repair taking ∼3-5 d. Skin repair after wounding is slower (∼2 wk). In the case of chronic wounds, it takes months to years to restore integrity. Herein, the unique "rapid-repair" endometrial environment is translated to the "slower repair" skin environment. Menstrual fluid (MF), the milieu of postmenstrual endometrial repair, facilitates healing of endometrial and keratinocyte "wounds" in vitro, promoting cellular adhesion and migration, stimulates keratinocyte migration in an ex vivo human skin reconstruct model, and promotes re-epithelialization in an in vivo porcine wound model. Proteomic analysis of MF identified a large number of proteins: migration inhibitory factor, neutrophil gelatinase-associated lipocalin, follistatin like-1, chemokine ligand-20, and secretory leukocyte protease inhibitor were selected for further investigation. Functionally, they promote repair of endometrial and keratinocyte wounds by promoting migration. Translation of these and other MF factors into a migration-inducing treatment paradigm could provide novel treatments for tissue repair.-Evans, J., Infusini, G., McGovern, J., Cuttle, L., Webb, A., Nebl, T., Milla, L., Kimble, R., Kempf, M., Andrews, C. J., Leavesley, D., Salamonsen, L. A. Menstrual fluid factors facilitate tissue repair: identification and functional action in endometrial and skin repair.

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Section: Identification Of Proteins In Mf Vs Matched Pb Plasma: Protmentioning

confidence: 85%

Menstrual fluid factors facilitate tissue repair: identification and functional action in endometrial and skin repair

et al. 2018

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“…Briefly, a 60-μg aliquot of each sample will be digested by trypsin, desalted, concentrated, and analysed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in data-independent acquisition mode for peptide identification and SWATH™ acquisition mode for peptide abundance. An existing peptide spectral ion library [125] will be used to identify the peptide products and generate proteomic profiles of the microenvironments of the different wounds. These profiles will be compared against one another as well as the proteomic profiles of paediatric burn blister fluid described previously by Zang et al [125, 126]…”

Section: Methodsmentioning

confidence: 99%

Study of negative pressure wound therapy as an adjunct treatment for acute burns in children (SONATA in C): protocol for a randomised controlled trial

Frear

Griffin

Cuttle

et al. 2019

Trials

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Background Although negative pressure wound therapy (NPWT) is widely used in the management of several wound types, its efficacy as a primary therapy for acute burns has not yet been adequately investigated, with research in the paediatric population particularly lacking. There is limited evidence, however, that NPWT might benefit children with burns, amongst whom scar formation, wound progression and pain continue to present major management challenges. The purpose of this trial is to determine whether NPWT in conjunction with standard therapy accelerates healing, reduces wound progression and decreases pain more effectively than standard treatment alone. Methods/design A total of 104 children will be recruited for this trial. To be eligible, candidates must be under 17 years of age and present to the participating children’s hospital within 7 days of their injury with a thermal burn covering <5% of their total body surface area. Facial and trivial burns will be excluded. Following a randomised controlled parallel design, participants will be allocated to either an active control or intervention group. The former will receive standard therapy consisting of Acticoat™ and Mepitel™. The intervention arm will be treated with silver-impregnated dressings in addition to NPWT via the RENASYS TOUCH™ vacuum pump. Participants’ dressings will be changed every 3 to 5 days until their wounds are fully re-epithelialised. Time to re-epithelialisation will be studied as the primary outcome. Secondary outcomes will include pain, pruritus, wound progression, health-care-resource use (and costs), ease of management, treatment satisfaction and adverse events. Wound fluid collected during NPWT will also be analysed to generate a proteomic profile of the burn microenvironment. Discussion The study will be the first randomised controlled trial to explore the clinical effects of NPWT on paediatric burns, with the aim of determining whether the therapy warrants implementation as an adjunct to standard burns management. Trial registration Australian New Zealand Clinical Trials Registry, ACTRN12618000256279 . Registered on 16 February 2018. Electronic supplementary material The online version of this article (10.1186/s13063-019-3223-9) contains supplementary material, which is available to authorized users.

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“…Methodology for blister fluid sample collection, sample preparation, liquid chromatography tandem mass spectrometry analysis, protein identification and GO analysis are described elsewhere [1] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…Detected proteins within the two over-represented GO terms, ‘ wound healing ’ and ‘ response to stress ’ were compared across three burn depths ( Tables A1 and B1 , respectively). Subsets of these data with additional interpretation relevant to burn injury can be found elsewhere [1] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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Mass spectrometry based data of the blister fluid proteome of paediatric burn patients

et al. 2016

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The data presented here are associated with the article “The blister fluid proteome of paediatric burns” (Zang et al., 2016) [1]. Burn injury is a highly traumatic event for children. The degree of burn severity (superficial-, deep-, or full-thickness injury) often dictates the extent of later scar formation which may require long term surgical operation or skin grafting. The data were obtained by fractionating paediatric burn blister fluid samples, which were pooled according to burn depth and then analysed using data dependent acquisition LC–MS/MS. The data includes a table of all proteins identified, in which burn depth category they were found, the percentage sequence coverage for each protein and the number of high confidence peptide identifications for each protein. Further Gene Ontology enrichment analysis shows the significantly over-represented biological processes, molecular functions, and cellular components of the burn blister fluid proteome. In addition, tables include the proteins associated with the biological processes of “wound healing” and “response to stress” as examples of highly relevant processes that occur in burn wounds.

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The blister fluid proteome of paediatric burns

Cited by 10 publications

References 44 publications

Menstrual fluid factors facilitate tissue repair: identification and functional action in endometrial and skin repair

Menstrual fluid factors facilitate tissue repair: identification and functional action in endometrial and skin repair

Study of negative pressure wound therapy as an adjunct treatment for acute burns in children (SONATA in C): protocol for a randomised controlled trial

Mass spectrometry based data of the blister fluid proteome of paediatric burn patients

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