No abstract

No abstract

No abstract

No abstract

No abstract

Abstract:The essential oil of the leaves from Annona coriacea Mart., Annonaceae, was extracted by hydrodistillation in a Clevenger apparatus and analyzed by GC/MS and GC/FID. The oil yield was 0.05% m/m. Sixty compounds were identified, in a complex mixture of sesquiterpenes (76.7%), monoterpenes (20.0%) and other constituents (3.3%). Bicyclogermacrene was its major compound (39.8%) followed by other sesquiterpenes. Most of the monoterpenes were in low concentration (<1%). Only β-pinene and pseudolimonene presented the highest level of 1.6%. The volatile oil presented anti-leishmanial and trypanocidal activity against promastigotes of four species of Leishmania and trypomastigotes of Trypanosoma cruzi, showing to be more active against Leishmania (L.) chagasi (IC50 39.93 µg/mL) (95% CI 28.00-56.95 µg/mL).

No abstract

IntroductionPropolis (also referred to as bee's glue) possesses a broad spectrum of biological activities, such as anti-hepatotoxic, antitumor, antiviral, antioxidant, antimicrobial and antiinflammatory properties, and is therefore used as a constituent of health foods and functional foods (BURDOCK, 1998).Propolis is collected from the leaf buds of numerous tree species (alder, birch, palm, pine, poplar and willow). Other plant exudates and secretions, such as substances secreted by plants to seal wounds, lipophylic substances on leaves, mucilages, gums, resins, lattices, etc. are also used (BANKOVA; CASTRO;MARCUCCI, 2000). Brazilian Green Propolis is collected from the tender sprouts of Baccharis dracunculifolia, which is commonly found in the States of Minas Gerais, São Paulo, Rio de Janeiro and Paraná AGUIAR, 2002;IKEGAKI;ALENCAR, 2000). In Brazil, twelve distinct groups of propolis have been classified according to their botanical origin and biological properties. Brazilian Green Propolis is classified as Group 12, which is the most commercialized and largely used in foods and beverages to improve health and prevent diseases (PARK et al., 2005).Propolis is in general composed of 50% resin and balsam, 30% wax, 10% essential and aromatic oils, 5% pollen, and 5% other substances, such as aliphatic acids, esters, aromatic acids, fatty acids, carbohydrates, aldehydes, amino acids, ketones, chalcones, dihydrochalcones, terpenoids, vitamins (B1, B2, B6, C and E) and minerals (aluminum, antimony, calcium, cesium, copper, iron, lanthanum, manganese, mercury, nickel, silver, vanadium and zinc) (ALMEIDA; MENEZES, 2002). Resumo Extratos etanólicos e óleos essenciais de própolis verde do sudeste brasileiro e gemas de sua origem botânica (Baccharis dracunculifolia) foram analisados por CLAE-FR (Cromatografia Líquida de Alta Eficiência em Fase Reversa), CCDAE (Cromatografia em Camada Delgada de Alta Eficiência) e CG-EM (Cromatografia Gasosa acoplada a Espectrometria de Massas). Os óleos essenciais foram obtidos por hidro destilação.Extratos etanólicos e óleos essenciais de Baccharis dracunculifolia e de própolis mostraram perfis cromatográficos similares entre si. Treze flavonóides foram identificados por CLAE-FR e CCDAE em ambas as amostras. Vinte e três compostos voláteis foram identificados por CG-EM, sendo dezessete deles presentes em ambos os óleos essenciais. Artepillin C foi o flavonóide encontrado em maiores concentrações em ambas as amostras, enquanto nerolidol foi o volátil majoritário em ambos os óleos essenciais. Os compostos majoritários identificados neste trabalho podem ser utilizados como marcadores químicos para classificar de forma prática e identificar origens botânicas de própolis. Palavras-chave: composição de óleos essenciais; Baccharis dracunculifolia; própolis; Alecrim do campo; flavonóides. AbstractEthanolic extracts and essential oils from Green Propolis from southeastern Brazil and leaf buds from its botanical origin Baccharis dracunculifolia were analyzed by Reversed Phase High Performance Liqui...