RegeneRação in vitRo deRESUMO Passiflora miniata é uma espécie silvestre nativa da Amazônica Meridional, com potencial ornamental devido à beleza de suas flores de coloração vermelha intensa. Relatos na literatura sobre a espécie ainda são insipientes. O objetivo do presente estudo foi induzir a regeneração de novo pela via organogênica a partir de embriões zigóticos maduros de P. miniata. Os embriões zigóticos foram isolados e cultivados no meio de MS com a adição dos reguladores de crescimento 6-Benziladenine (BA), Thidiazuron (TDZ) e Cinetina (CIN). A regeneração de novo nos embriões zigóticos ocorreu de forma direta e/ou indireta. Um percentual de 80% dos explantes cultivados na presença de BA apresentaram organogênese pela via direta e 20% pela indireta, com TDZ 60% foram regenerados pela via direta e 40% pela indireta. Já nos tratamentos com CIN 58% do explantes apresentaram regeneração por organogênese direta e 42% pela indireta. O desenvolvimento dos primórdios das brotações iniciou-se com a formação das estruturas organogênicas que posteriormente se diferenciaram em multibrotações. As maiores médias no número de brotos produzidos foram na concentração de 0,75 mg L -1 de BA com 40,0 de brotos por explantes, sendo TDZ e CIN na concentração de 0,50 mg L -1 com média de 7,2 e 3,6, respectivamente. Palavras chaves: Passiflora miniata, Maracujá Amazônico, Embrião zigótico, Organogênese. ABSTRACT In vitro regeneration of Passiflora miniata MastPassiflora miniata is a wild species native to the Southern Amazon, with ornamental potential due to the beauty of its flowers of intense red coloration. Reports in the literature about the species are still insipid. The aim of the present study was to induce the regeneration of P. miniata by the de novo organogenesis from mature zygotic embryos. The zygotic embryos were isolated and cultivated into the MS medium with the addition of 6-Benzyladenine (BA), Thidiazuron (TDZ) and Kinetin (KIN) growth regulators. The de novo regeneration from the zygotic embryos occurred directly and indirectly. A percentage of 80% of the explants cultivated in the presence of BA had direct organogenesis and 20% by the indirect way, with TDZ 60% were regenerated by the direct and 40% by the indirect way. Regarding the treatments with KIN, 58% of the explants had regeneration by direct and 42% by the indirect organogenesis. The development of shoot primordia initiated with the formation of organogenic structures that later differentiated into multi-shoots. The highest mean number of shoots (40.0 shoots per explants) was obtained on 0.75 mg L -1 BA. Conversely, using 0.50 mg L -1 TDZ or KIN, the highest number of shoots were 7.2 and 3.6, respectively.
range 2-9 µM) of 6-benzyladenine (BA); thidiazuron (TDZ) or kinetin (KIN). Plant growth regulators were not added to the control treatment. Root explants have showed a high regenerative potential. After 30 days of in vitro culture, the root explants showed several shoots formed direct and indirectly. TDZ provided the best response in the differentiation adventitious shoots, mainly in the presence of 6.8 µM. The cytokinins BA and KIN responded producing a reduced number of shoots. After 120 days, rooted regenerated plants were transferred to a greenhouse for acclimatization. This regeneration system opens new perspectives for micropropagation and conservation of this wild Amazonic passion fruit species.
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