Regeneração in vitro de Passiflora miniata Mast

Abstract

RegeneRação in vitRo deRESUMO Passiflora miniata é uma espécie silvestre nativa da Amazônica Meridional, com potencial ornamental devido à beleza de suas flores de coloração vermelha intensa. Relatos na literatura sobre a espécie ainda são insipientes. O objetivo do presente estudo foi induzir a regeneração de novo pela via organogênica a partir de embriões zigóticos maduros de P. miniata. Os embriões zigóticos foram isolados e cultivados no meio de MS com a adição dos reguladores de crescimento 6-Benziladenine (BA), Thidiazuron (TDZ) e Cinetina (CIN). A regeneração de novo nos embriões zigóticos ocorreu de forma direta e/ou indireta. Um percentual de 80% dos explantes cultivados na presença de BA apresentaram organogênese pela via direta e 20% pela indireta, com TDZ 60% foram regenerados pela via direta e 40% pela indireta. Já nos tratamentos com CIN 58% do explantes apresentaram regeneração por organogênese direta e 42% pela indireta. O desenvolvimento dos primórdios das brotações iniciou-se com a formação das estruturas organogênicas que posteriormente se diferenciaram em multibrotações. As maiores médias no número de brotos produzidos foram na concentração de 0,75 mg L -1 de BA com 40,0 de brotos por explantes, sendo TDZ e CIN na concentração de 0,50 mg L -1 com média de 7,2 e 3,6, respectivamente. Palavras chaves: Passiflora miniata, Maracujá Amazônico, Embrião zigótico, Organogênese. ABSTRACT In vitro regeneration of Passiflora miniata MastPassiflora miniata is a wild species native to the Southern Amazon, with ornamental potential due to the beauty of its flowers of intense red coloration. Reports in the literature about the species are still insipid. The aim of the present study was to induce the regeneration of P. miniata by the de novo organogenesis from mature zygotic embryos. The zygotic embryos were isolated and cultivated into the MS medium with the addition of 6-Benzyladenine (BA), Thidiazuron (TDZ) and Kinetin (KIN) growth regulators. The de novo regeneration from the zygotic embryos occurred directly and indirectly. A percentage of 80% of the explants cultivated in the presence of BA had direct organogenesis and 20% by the indirect way, with TDZ 60% were regenerated by the direct and 40% by the indirect way. Regarding the treatments with KIN, 58% of the explants had regeneration by direct and 42% by the indirect organogenesis. The development of shoot primordia initiated with the formation of organogenic structures that later differentiated into multi-shoots. The highest mean number of shoots (40.0 shoots per explants) was obtained on 0.75 mg L -1 BA. Conversely, using 0.50 mg L -1 TDZ or KIN, the highest number of shoots were 7.2 and 3.6, respectively.

Tissue culture and biotechnological techniques applied to passion fruit with ornamental potential: an overview

“…For Passiflora miniata, an Amazonian species with great ornamental potential, a regeneration system was observed via organogenesis from zygotic embryos. Regeneration occurred directly and/or from calluses, mainly in the presence of 3.32 µM BA, with an average of 40.0 shoots per explant (Carvalho et al, 2017). Recently, Faria and collaborators (2018) highlighted the high responsiveness of Passiflora cristalina, another Amazonian species with ornamental potential.…”

“…These flowers are found in the most diverse forms, sizes, and colors and have a distinguished scent (Montero et al, 2013). In the view of the recognized economic importance of the wild and commercial passion fruit species several tissue culture and biotechnological techniques have been applied to the genus (Silva et al, 2009;Otoni et al, 2013;Ozarowski and Thiem 2013;Pacheco et al, 2016;Carvalho et al, 2017;Faria et al, 2018). The present review proposes to summarize the results achieved thus far in the biotechnology of wild Passiflora species with ornamental potential.…”

In Vitro Organogenesis from Root Explants of Passiflora miniata Mast., an Amazonian Species with Ornamental Potential

“…Regeneration protocols have been established from different types of explants, e.g. leaf, hypocotyl, root, nodal and intermodal segments and zygotic embryos [5][6][7][8][9][10][11][12][13]. At present, root segments have been adopted as explant source for in vitro regeneration of passion fruit [14,15,11,16]; for being easily obtained and maintained under in vitro conditions, in general [17].…”

“…This species has been extensively cultivated erroneously as Passiflora coccinea because of similar characteristics such as bright-red flowers [20]. Studies involving the in vitro regeneration of P. miniata have only been published by [21,13], were established a protocol involving somatic embryogenesis and organogenesis using zygotic embryos as explant sources, respectively. In the present study, we report the establishment of a protocol for in vitro regeneration of P. miniata via organogenesis from root segments.…”

Agronomic descriptors and ornamental potential of passion fruit species

“…presents great genetic variability. The genus is composed of approximately 530 species, of which 150 to 200 are native to Brazil, with about 70 species that produce edible fruits (CARVALHO et al, 2017;FALEIRO et al, 2005). Brazil is one of the most important centers of passion flower diversity since most of wild Passiflora species occur in the center-north region of the country (FALEIRO et al, 2011).…”