The influence of the sludge loading rate, the addition of a small amount of granular sludge to the seed sludge and the effect of the NH4+- and Ca2+-concentration in the influent on the pelletization-process of anaerobic methanogenic sludge in UASB-reactors were studied. Two different types of sludge granules can be obtained with VFA's as feed-solution.
Isolation and characterization of a new, obligatory, anaerobic, methylotrophic, homoacetogenic bacterium is described. This bacterium is a mesophilic, motile, slightly curved rod that demonstrated a negative Gram reaction, formed spherical, (sub)terminal spores and performed a homoacetic fermentation with methanol, a CO2-2H2-gas mixture, glucose or fructose, respectively, as the substrate. The methanol fermentation proceeded only when a suitable amount of NaHCO3 was available in the nutrient solution supplied.
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