Features of a Clostridium, strain CV-AA1, an obligatory anaerobic bacterium producing acetic acid from methanol

Adamse, A. D.; Velzeboer, C. T. M.

doi:10.1007/bf00418284

Cited by 26 publications

(6 citation statements)

References 15 publications

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“…2). The onset of reduction was significantly delayed for methanol‐amended microcosms by as much as 15 d. Slight decreases in the U(VI) concentrations between days 12 and 28 were likely due to decreases in pH from fermentation reactions, such as 4CH 3 OH + 2HCO 3 − = 3CH 3 COO − + H + + 4H 2 O (Adamse and Velzeboer, 1982), that produce acetate and other low‐molecular weight organic acids. For all donor‐amended microcosms, the total aqueous U(VI) concentrations reached relatively steady values after 34 d (ethanol and 5 mmol L −1 glucose) or 42 d (methanol, methanol with humic acids) in the range of 0.4 to 1 μmol L −1 After the period of rapid reduction of most aqueous U(VI), concentrations continued to fall slowly over the period of 42 to 92 d, reaching final concentrations below the EPA MCL of 30 ppb.…”

Section: Resultsmentioning

confidence: 99%

Donor‐dependent Extent of Uranium Reduction for Bioremediation of Contaminated Sediment Microcosms

et al. 2009

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Bioremediation of uranium was investigated in microcosm experiments containing contaminated sediments from Oak Ridge, Tennessee to explore the importance of electron donor selection for uranium reduction rate and extent. In these experiments, all of the electron donors, including ethanol, glucose, methanol, and methanol with added humic acids, stimulated the reduction and immobilization of aqueous uranium by the indigenous microbial community. Uranium loss from solution began after the completion of nitrate reduction but essentially concurrent with sulfate reduction. When electron donor concentrations were normalized for their equivalent electron donor potential yield, the rates of uranium reduction were nearly equivalent for all treatments (0.55-0.95 micromol L(-1) d(-1)). Uranium reduction with methanol proceeded after a 15-d longer lag time relative to that of ethanol or glucose. Significant differences were not found with the inclusion of humic acids. The extent of U reduction in sediment slurries measured by XANES at various time periods after the start of the experiment increased in the order of ethanol (5-7% reduced at 77 and 153 d), glucose (49% reduced at 53 d), and methanol (93% reduced at 90 d). The microbial diversity of ethanol- and methanol-amended microcosms in their late stage of U reduction was analyzed with 16S rRNA gene amplification. Members of the Geobacteraceae were found in all microcosms as well as other potential uranium-reducing organisms, such as Clostridium and Desulfosporosinus. The effectiveness of methanol relative to ethanol at reducing aqueous and sediment-hosted uranium suggests that bioremediation strategies that encourage fermentative poising of the subsurface to a lower redox potential may be more effective for long-term uranium immobilization as compared with selecting an electron donor that is efficiently metabolized by known uranium-reducing microorganisms.

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Section: Resultsmentioning

confidence: 99%

Donor‐dependent Extent of Uranium Reduction for Bioremediation of Contaminated Sediment Microcosms

et al. 2009

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“…The isolated acetogenic strain can also use a mixture of carbon dioxide and hydrogen as a substrate for acetic acid production. Also in this case addition of bicarbonate stimulated acetogenesis (Adamse and Velzeboer, 1982).…”

Section: Microbiology Of Anaerobic Waste Conversionmentioning

confidence: 98%

“…Bacteria which do not form methane but are able to grow on methanogenic substrates (e.g. homoacetogens) have in recent years attracted the interest of an increasing number op scientists (Balch et al, 1977;Adamse, 1980;Braun et al, 1981;Adamse and Velzeboer, 1982;Braun and Gottschalk, 1982;Zeikus, 1983). Their quantitative and qualitative role in anaerobic digestion is, however, not yet understood.…”

Section: Microbiology Of Anaerobic Waste Conversionmentioning

confidence: 99%

Studies on bacterial activities in aerobic and anaerobic waste water purification

Adamse

Deinema

Zehnder

1984

Antonie van Leeuwenhoek

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Some aspects of the bacteriology of aerobic and anaerobic waste water purification are discussed in view of current opinions and recent developments in the technology of waste water treatment. Various contributions of scientific workers attached to the Department of Microbiology of the Agricultural University, Wageningen, during the past 65 years are summarized. Besides, present investigations are described and research activities in future indicated.

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“…During the past 6 years, several new types of obligately anaerobic bacteria have been discovered " "Fo whom correspondence should be addressed. that carry' out acidogenic fermentation of methanol and CO, [1][2][3][4][5][6]. A fermentation of this type has also been rcported for a few species that had been described earlicr, but whose methylotrophic potential had not previously been revealed [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Interspecies hydrogen transfer in co-cultures of methanol-utilizing acidogens and sulfate-reducing or methanogenic bacteria

Heijthuijsen

Hansen

1986

FEMS Microbiology Letters

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The metabolism of methanol by acidogenic bacteria (Butyribacterium methylotrophicum, Sporomusa ovata and Acetobacterium woodii) was studied in pure culture and in defined mixed cultures with sulfate‐reducing bacteria (Desulfovibrio vulgaris) or methanogenic bacteria (Methanobrevibacter arboriphilus strain AZ). In the mixed cultures, less acids (acetate and/or butyrate) were formed per unit methanol converted than in pure cultures. In these mixed cultures, a significant production of sulfide or methane was observed despite the inability of the sulfate reducer and the methanogen to use methanol as an energy substrate. These results are explained in terms of interspecies hydrogen transfer between the acidogens (converting part of the methanol to 1 CO2 and 3 H2) and the Desulfovibrio or Methanobrevibacter species. The bioenergetic aspects of this process and its ecological implications are discussed.

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Features of a Clostridium, strain CV-AA1, an obligatory anaerobic bacterium producing acetic acid from methanol

Cited by 26 publications

References 15 publications

Donor‐dependent Extent of Uranium Reduction for Bioremediation of Contaminated Sediment Microcosms

Donor‐dependent Extent of Uranium Reduction for Bioremediation of Contaminated Sediment Microcosms

Studies on bacterial activities in aerobic and anaerobic waste water purification

Interspecies hydrogen transfer in co-cultures of methanol-utilizing acidogens and sulfate-reducing or methanogenic bacteria

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