A method for analysis of ergovaline in seed and vegetative tissue of endophyte-infested tall fescue by high-performance liquid chromatography with fluorescence detection was developed. Freeze-dried samples were extracted with alkaline chloroform, filtered, and applied to disposable HL silica gel columns. Following elution of plant pigments with acetone/chloroform, ergopeptine alkaloids were eluted with methanol under vacuum. Column eluant was evaporated to near dryness and the residue redissolved in methanol/water and analyzed by high-performance liquid chromatography; ergotamine was the internal standard. Average recoveries of ergovaline and ergotamine were 85 and 88%, respectively, with a relative standard deviation of 7.5%. The detection limit of ergovaline in fescue seed and vegetative tissue was 50 pg/kg. Replicate samples, collected each week during two growing seasons from experimental plots of KY-31 tall fescue (85-100'3 endophyte-infested), were separated into stems with leaf sheaths, leaf blades, and developing seed heads. Ergovaline levels in all plant tissues were 300-

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Swine with a hemophilia-like disease characterized by a long bleeding time and low antihemophilic factor (AHF, factor VIII) level were infused with porcine plasma and porcine serum. Unlike the response of classical hemophiliacs, but similar to that of patients with von Willebrand's disease, the initial postinfusion levels of AHF were sustained with some fluctuation for 24 hr. Serum infusion resulted in a delayed increase in AHF which reached a maximum concentration between the 12th and 24th hr after infusion. Within 75 hr the effects of both the plasma and serum infusions had vanished. No correction of the prolonged bleeding times was observed following the infusions. The AHF levels encountered after the infusion of plasma and serum cannot be accounted for by the AHF activity contained in the infused materials; new AHF synthesis or AHF release from storage are suggested as possible mechanisms.

Digital angiography of the caprine hindlimb was done via catheterization of dorsal metatarsal artery III. Good arteriograms were consistently produced. The vascular anatomy to the digit was similar to that reported in previous bovine studies. The caprine may be of use as a model for selected ruminant foot disease.