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The enzyme specificity in the biosynthesis of prostaglandins has been investigated by incubation of a particulate fraction of sheep vesicular glands with w6 tri-and tetraenoic CIS-CZZ fatty acids and isomers of w6 eicosatrienoic acid (20 : 3 06). The chemical synthesis of the various substrate fatty acids is described. The tetraenoic fatty acids were converted into a series of prostaglandin Ez homologues and the trienoic fatty acids into a series of prostaglandin El homologues. The only isomer of 20 : 3 w6 which showed a fairly high conversion into an isoprostaglandin E l was the 0 7 compound. Maximum conversions were obtained from w6 CZO acids.The smooth muscle stimulating activity of prostaglandin EL and Ez appears to be very specific. All other prostaglandins had much lower activities.

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