The aim: To investigate the informative content of immunocytochemical studies of lymph node aspirate using a molecular marker of cytokeratin 19 in patients with laryngeal
cancer as for regional metastastatic spread.
Materials and methods: A comprehensive investigation with immunohistochemical study of squamous cell carcinoma of larynx of 74 patients with laryngeal cancer II – IV
stage (T2-4 N0-3 M0) of the second clinical group was conducted.
Results: Statistically significant differences (p <0.001) in the structure of distribution of marker expression between groups were revealed. Thus, a marked expression of
cytokeratin 19 was determined in 11 (29.73%) representatives of the group with regional metastases and only in 1 (2.7%) patient without tumor metastases (p = 0.002). In
the group with metastases the presence of atypical cells was confirmed cytologically in 26 cases (70,27%).
Conclusions: To predict the development of laryngeal metastases in laryngeal cancer patients, it is recommended to carry out an immunocytochemical study using a diagnostic
marker of cytokeratin 19. The positive status of cytokeratin 19 increases the chances of regional metastasis of laryngeal cancer by 18.37 times (p<0.001) compared with absence
of expression.
In this study we investigated the expression levels of molecular markers of matrix metalloproteinase 2 and matrix metalloproteinase 9 in patients with larynx cancer with regional metastases. Cytological specimens were examined by means of immunocytochemical tecnique. The material was obtained by fine-needle aspiration biopsy. A relationship between the expression of the marker and the presence of metastases has been established. A link between the expression of molecular markers of matrix metalloproteinase 2 and matrix metalloproteinase 9 with metastasis to lymph nodes in a direct correlation has been proved which allows us to recommend the study of these markers as factors in the metastatic squamous cell carcinoma of the larynx in the regional lymph nodes in the neck.
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