Objective. To learn the expression level of cell adhesion markers (E-cadherin and β-catenin) in oropharyngeal squamous cell carcinoma (OSCC) for establishing clinical and morphological connections and analyzing their prognostic meaning of relation with regional metastases.Design. Expression of E-cadherin and β-catenin, epithelial cell adhesion molecules was examined by using immunohistochemical analysis (E-cadherin, clon EP700Y, Lab Vision, β-catenin, clon E247, Lab Vision and visualization system EnVision, Dako Cytomation), both traditional histological parameters and the correlation of these markers with the histologically verified presence of regional metastases have been determined.Subjects. The study of 72 patients was comprised with OSCC. They were divided into two groups with regional metastases (T 3 N 1-3 M 0 ) -38 patients and without metastases (T 3 N 0 M 0 ) -34 patients. Paraffinembedded material of primary tumors was used.Results. Obtained information is presented in the table of markers` indexes expression. The difference of E-cadherin expression markers is established in groups with and without metastases. Differences between groups are significant statistically (p<0.001). The reliable indirect correlated connection has been established between these parameters (r=+0,471). The difference of β-catenin expression markers is established in groups with and without metastases. Differences between groups are significant statistically (p=0,002). The reliable indirect correlated connection has been established between these parameters (r=+0,373). It gives the opportunity to assert the practicability of E-cadherin and β-catenin markers usage to prognosis the meaning of relation with regional metastases.Conclusions. The expression levels of intercellular adhesion markers (E-cadherin and β-catenin) have been investigated with OSCC patients to identify clinical and morphological connections and analyze their prognostic meaning of relation with regional metastases. Markers relative predictive values of regional metastasis have been established. These results can be used for making decision on the subject of the patients` treatment of OSCC.
The aim: To investigate the informative content of immunocytochemical studies of lymph node aspirate using a molecular marker of cytokeratin 19 in patients with laryngeal cancer as for regional metastastatic spread. Materials and methods: A comprehensive investigation with immunohistochemical study of squamous cell carcinoma of larynx of 74 patients with laryngeal cancer II – IV stage (T2-4 N0-3 M0) of the second clinical group was conducted. Results: Statistically significant differences (p <0.001) in the structure of distribution of marker expression between groups were revealed. Thus, a marked expression of cytokeratin 19 was determined in 11 (29.73%) representatives of the group with regional metastases and only in 1 (2.7%) patient without tumor metastases (p = 0.002). In the group with metastases the presence of atypical cells was confirmed cytologically in 26 cases (70,27%). Conclusions: To predict the development of laryngeal metastases in laryngeal cancer patients, it is recommended to carry out an immunocytochemical study using a diagnostic marker of cytokeratin 19. The positive status of cytokeratin 19 increases the chances of regional metastasis of laryngeal cancer by 18.37 times (p<0.001) compared with absence of expression.
In this study we investigated the expression levels of molecular markers of matrix metalloproteinase 2 and matrix metalloproteinase 9 in patients with larynx cancer with regional metastases. Cytological specimens were examined by means of immunocytochemical tecnique. The material was obtained by fine-needle aspiration biopsy. A relationship between the expression of the marker and the presence of metastases has been established. A link between the expression of molecular markers of matrix metalloproteinase 2 and matrix metalloproteinase 9 with metastasis to lymph nodes in a direct correlation has been proved which allows us to recommend the study of these markers as factors in the metastatic squamous cell carcinoma of the larynx in the regional lymph nodes in the neck.
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