Analysis of Сytoceratine 19 Expression Level By Immunocytochemical Diagnostic Method in Laryngeal Cancer Patients With Regional Metastases

Abstract

The aim: To investigate the informative content of immunocytochemical studies of lymph node aspirate using a molecular marker of cytokeratin 19 in patients with laryngeal cancer as for regional metastastatic spread. Materials and methods: A comprehensive investigation with immunohistochemical study of squamous cell carcinoma of larynx of 74 patients with laryngeal cancer II – IV stage (T2-4 N0-3 M0) of the second clinical group was conducted. Results: Statistically significant differences (p <0.001) in the structure of distribution of marker expression between groups were revealed. Thus, a marked expression of cytokeratin 19 was determined in 11 (29.73%) representatives of the group with regional metastases and only in 1 (2.7%) patient without tumor metastases (p = 0.002). In the group with metastases the presence of atypical cells was confirmed cytologically in 26 cases (70,27%). Conclusions: To predict the development of laryngeal metastases in laryngeal cancer patients, it is recommended to carry out an immunocytochemical study using a diagnostic marker of cytokeratin 19. The positive status of cytokeratin 19 increases the chances of regional metastasis of laryngeal cancer by 18.37 times (p<0.001) compared with absence of expression.