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Andrographolide, the active constituent, isolated from the plant Andrographis paniculata showed concentration dependent (1-100 microg/mL) activity against galactosamine (GALN 400 microg/mL) induced acute injury in isolated monkey hepatocytes, following 48 hours of the incubation at 370 C. The effect of andrographolide was measured on viability (trypan blue exclusion and oxygen uptake tests), biochemical marker enzymes -aspartate amino transaminase (AST), alanine amino transaminase (ALT) and alkaline phosphatase (AlP) and bile contents (bile salts and bile acids). It significantly increased the percent viability of cells and was capable of preserving 90-100% cell integrity. The cellular leakage of enzymes and bile contents following incubation with GALN was significantly modulated by andrographolide treatment. Its activity was found superior to that of silymarin, a standard hepatoprotective compound, tested simultaneously for comparison. The findings also emphasize the possible use of an in-vitro primary cell culture system as an alternative to in-vivo, in the early stage of drug discovery.