No abstract

The adaptations of root morphology, physiology, and biochemistry to phosphorus supply have been characterized intensively. However, characterizing these adaptations at molecular level is largely neglected under field conditions. Here, two consecutive field experiments were carried out to investigate the agronomic traits and root traits of wheat (Triticum aestivum L.) at six P-fertilizer rates. Root samples were collected at flowering to investigate root dry weight, root length density, arbusular-mycorrhizal colonization rate, acid phosphatase activity in rhizosphere soil, and expression levels of genes encoding phosphate transporter, phosphatase, ribonucleases, and expansin. These root traits exhibited inducible, inhibitory, or combined responses to P deficiency, and the change point for responses to P supply was at or near the optimal P supply for maximum grain yield. This research improves the understanding of mechanisms of plant adaptation to soil P in intensive agriculture and provides useful information for optimizing P management based on the interactions between soil P dynamics and root processes.

Decreased streamflow of the Yellow River basin has become the subject of considerable concern in recent years due to the critical importance of the water resources of the Yellow River basin for northern China. This study investigates the changing properties and underlying causes for the decreased streamflow by applying streamflow data for the period 1960 to 2014 to both the Budyko framework and the hydrological modelling techniques. The results indicate that (1) streamflow decreased 21% during the period 1980-2000, and decreased 19% during the period 2000-2014 when compared to 1960-1979; (2) higher precipitation and relative humidity boost streamflow, while maximum/minimum air temperature, solar radiation, wind speed, and the underlying parameter, n, all have the potential to adversely affect them; (3) decreased streamflow is also linked to increased cropland, grass, reservoir, urban land, and water areas and other human activities associated with GDP and population; (4) human activity is the main reason for the decrease of streamflow in the Yellow River basin, with the mean fractional contribution of 73.4% during 1980-2000 and 82.5% during 2001-2014. It is clear that the continuing growth of human-induced impacts on streamflow likely to add considerable uncertainty to the management of increasingly scarce water resources. Overall, these results provide strong evidence to suggest that human activity is the key factor behind the decreased streamflow in the Yellow River basin.

Land reclamation may expand the supply of usable land for food security. Immature soil is not suitable for plant growth and needs to be amended by the addition of organic matter and plant growth-promoting (PGP) microorganisms. However, the effects of different PGP fungi on plant growth in immature soil are largely unexplored. In order to obtain beneficial soil microorganisms with a good PGP ability in new reclamation land, 162 fungal isolates were isolated from different abandoned wastelands, four isolates of which were obtained in this study by the screening of P solubilization, siderophore production, and indole acetic acid (IAA) production. The result of this study revealed that isolate HZ123 had the highest ability to solubilize P and produce siderophores and IAA, followed by HZ23, HZ10, and HZ06. Based on the results of morphological and molecular analyses, isolate HZ06 was identified as Penicillium oxalicum, isolates HZ23 and HZ10 were identified as Aspergillus brunneoviolaceus, and isolate HZ123 was identified as Aspergillus tubingensis. Furthermore, the results of in vivo PGP assays demonstrated that isolate HZ123 has a minimal negative effect on the growth of eggplant; however, the other three isolates, particularly isolate HZ06, caused the greatest increase in eggplant biomasses. Overall, these results indicate that isolate HZ06 has great potential as a PGP fungus to develop biofertilizer for application in eggplant production in immature soil from new reclamation land.