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-chronic oral toxicity study in SpragueDawley rats with hypoxia mimetic cobalt chloride towards the development of promising neutraceutical for oxygen deprivation, Experimental and Toxicologic Pathology, doi:10.1016Pathology, doi:10. /j.etp.2009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 3. Biologicals E. Ltd. Hyderabad, India. *Corresponding Author A c c e p t e d m a n u s c r i p t 2 AbstractThe present study evaluates the toxicity from sub-chronic administration of CoCl 2 (12.5 mg cobalt kg -1 BW for seven days) to male Sprague-Dawley rats in view of the beneficial effects of CoCl 2 in animals and for developing efficacious therapeutic regimen in humans. 32 rats weighing 200±25g were used for all experiments. Blood was collected for hematological and biochemical analysis and various organs were dissected after perfusion of animals under anesthesia for other analyses. Mean feed consumption and feed conversion efficiency values were comparable across all study groups; however, Hematological analysis depicted a significant increase in hemoglobin, hematocrit and RBC in the entire cobalt supplemented groups which are a component of its beneficial effect. There was a significant increase in monocytes, Granulocytes and WBC after 1h and 24h which were comparable with control after 7 days. Other biochemical analyses also showed no change with respect to control. Though the metal content increased significantly in liver initially (1h and 24h) after treatment, it was equivalent to control after 7 days.Moreover, histopathological analysis revealed no evidence of changes that could be attributed to cobalt pretreatment. It is therefore reasonable to conclude that the present study supports further use of the present dose of CoCl 2 which was found to be nontoxic.