Haematococcus pluvialis was induced to produce the astaxanthin pigment. A factorial design was carried out with three sodium acetate concentrations, 0.025, 0.05, 0.1 (gfl), and three NaCl concentrations (0.1, 0.2 and 0.4 %).The best conditions in algal culture for astaxanthin production were 0.2 % NaCI, 0.025 g/l sodium acetate and 0.05 g/1 sodium acetate, each a 3.0, 1.83 and 1.78 % of astaxanthin, production in base to total dry weight, respectively. The higher astaxanthin production by bioreactor was 18.6 mg/] in the condition with 0.2 % NaCI.
A factorial experiment comprising six nutrient concentrations and six renewal rates was set to optimize semicontinuous cultures of Phaeodactylum tricornutum. Maximal cell density, 85 ؋ 10 6 cells ml ؊1 , was obtained with 8 and 16 mmol of N per liter and a renewal rate of 10%. Protein stabilized at about 60% of the organic fraction for nitrogen-sufficient cultures. Both lipids and carbohydrates were stored as energetic reserves.
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