Astaxanthin production from the green alga Haematococcus pluvialis with different stress conditions

Abstract

Haematococcus pluvialis was induced to produce the astaxanthin pigment. A factorial design was carried out with three sodium acetate concentrations, 0.025, 0.05, 0.1 (gfl), and three NaCl concentrations (0.1, 0.2 and 0.4 %).The best conditions in algal culture for astaxanthin production were 0.2 % NaCI, 0.025 g/l sodium acetate and 0.05 g/1 sodium acetate, each a 3.0, 1.83 and 1.78 % of astaxanthin, production in base to total dry weight, respectively. The higher astaxanthin production by bioreactor was 18.6 mg/] in the condition with 0.2 % NaCI.

Astaxanthin Level of Dominant Mangrove Floral Species in Indian Sundarbans

“…Many yeasts have been described with an increase ability to produce carotenoids when they grow under unfavorable environment [22]. The enhancement of the accumulation of astaxanthin in cysts of Haematococcus pluvialis under salt stress conditions has been reported both in the dark and light by several workers [23][24][25][26][27][28][29][30][31]. The present study has pointed higher astaxanthin level in those leaves of the mangroves that are inundated for 10 to 12 hours by tidal waters (ambient water salinity during the period of investigation was 8.50 o / oo in the northern tip of Sagar Island and 27.23 o / oo in the southern tip of Sagar island) thus exposed to stress caused by aquatic salinity.…”

Systemic Statistical Optimization of Astaxanthin Inducing Methods in Haematococcus pluvialis cells -Statistical Optimization of Astaxanthin Production in Haematococcus

“…Light intensity and nitrate were selected as effective factors from the FFD. Phosphate and acetate were included in CCD because they were known as induction factors for astaxanthin production [1, 4,10,14]. Central values of nitrate and phosphate were taken from the original concentration of MBBM while the acetate concentration was 0.2 g/L.…”

“…Of particular importance is depletion of the nutrient source in the medium [7,8]. Another report showed that N-deficiency [21] induced astaxanthin formation and C/N balance [12] in the medium determined the degree of carotene formation [4]. Phosphate starvation was reported previously to act as a trigger for the accumulation of astaxanthin [10].…”

“…N, C, and P are essential nutrients that determine the rate of synthesis and final concentration of astaxanthin. Metal ions, oxidative stress, and salt stress stimulate the formation of astaxanthin in this alga [1,4,10,25]. The ferrous form of iron is known to give rise to free radical formation via the Fenton reaction, and free radicals may play a role in astaxanthin formation.…”

Combined effects of nitrate concentration and illumination conditions on the growth of microalga Haematococcus pluvialis

“…Trong khi đó, vi tảo lục Haematococcus pluvialis có khả năng tích lũy astaxanthin lên tới trên 4% sinh khối khô [10]. Vì vậy, hiện nay sản xuất astaxanthin từ loài vi tảo này đang được đặc biệt quan tâm nghiên cứu [2, 5,6,13,19]. Tuy nhiên, việc sản xuất astaxanthin hiệu quả từ loài vi tảo này còn gặp nhiều khó khăn bởi vì chúng có tốc độ sinh trưởng thấp và nhạy cảm với sự thay đổi của điều kiện nuôi cấy.…”