Optimal Renewal Rate and Nutrient Concentration for the Production of the Marine Microalga Phaeodactylum tricornutum in Semicontinuous Cultures

Fábregas, Jaime; Patiño, Manuel R. Bermejo; Morales, Ever; Cordero, Beatriz; Otero, Ana

doi:10.1128/aem.62.1.266-268.1996

Cited by 31 publications

(7 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The copyright holder for this preprint this version posted June 23, 2021. ; https://doi.org/10.1101/2021.06.23.449330 doi: bioRxiv preprint culture and they accumulate to a high cell density. Indeed, P. tricornutum reached abundances significantly higher than previously reported values, including under (semi-) continuous systems [60,61]. We believe this is the first demonstration that algae can accumulate to such high densities, purely driven by a novel cultivation technique.…”

Section: Discussionsupporting

confidence: 51%

Bacterial response to spatial gradients of algal-derived nutrients in a porous microplate

Kim

Kimbrel

Vaiana

et al. 2021

Preprint

View full text Add to dashboard Cite

Photosynthetic microalgae are responsible for 50% of the global atmospheric CO2 fixation into organic matter and hold potential as a renewable bioenergy source. Their metabolic interactions with the surrounding microbial community (the algal microbiome) play critical roles in carbon cycling, but due to methodological limitations, it has been challenging to examine how community is developed by spatial proximity to their algal host. Here we introduce a hydrogel-based porous microplate to co-culture algae and bacteria, where metabolites are constantly exchanged between the microorganisms while maintaining physical separation. In the microplate we found that the diatom Phaeodactylum tricornutum accumulated to cell abundances ~20 fold higher than under normal batch conditions due to constant replenishment of nutrients through the hydrogel. We also demonstrate that algal-associated bacteria, both single isolates and complex communities, responded to inorganic nutrients away from their host as well as organic nutrients originating from the algae in a spatially predictable manner. These experimental findings coupled with a mathematical model suggest that host proximity and algal culture growth phase impact bacterial community development in a taxon-specific manner through organic and inorganic nutrient availability. Our novel system presents a useful tool to investigate universal metabolic interactions between microbes in aquatic ecosystems.

show abstract

Section: Discussionsupporting

confidence: 51%

Bacterial response to spatial gradients of algal-derived nutrients in a porous microplate

Kim

Kimbrel

Vaiana

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Repeated batch cultivation has proved to be an excellent method for raising microbial productivity,24, 25 adjusting the nutrients feed rate to optimize the productivity26 and maintaining the microorganism at high growth rate 27. Some researchers adopted this method to enhance hydrogen production in dark fermentation 28, 29…”

Section: Resultsmentioning

confidence: 99%

Thermo‐alkaline pretreatment of lipid‐extracted microalgal biomass residues enhances hydrogen production

Yang

Guo

et al. 2010

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: The conversion of lipid-extracted microalgal biomass residues (LMBRs) into hydrogen serves the dual role in renewable energy production and sustainable development of microalgal biodiesel industry. In this work, batch experiments were performed to convert LMBRs pretreated by a thermo-alkaline method into hydrogen. In addition, repeated batch cultivation was conducted using the pretreated LMBRs as feedstocks under optimal pretreatment condition to further obtain high hydrogen production.

show abstract

“…semicontinuous cultivation shows that the specific growth rate of the microalga was increased when the medium renewal rate increased. The authors have also found that, with renewal rates of 40 to 50%, the percentage of conversion of nitrogen in the cells was below 100%, indicating that there was no nitrogen limitation [ 10 ].…”

Section: Resultsmentioning

confidence: 99%

“…After a certain period of time, a portion of the culture medium with the microalgae is removed and replaced with fresh culture medium without cells [ 9 ]. This type of cultivation has operational advantages, such as dispensing with the constant changing of the inoculums and allowing the microorganism to grow at a high speed [ 10 ]. Although widely used, this system of cultivation has hardly been studied, and there is a lack of scientific work involving variables such as blend concentration and medium renewal rate.…”

Section: Introductionmentioning

confidence: 99%

Effect of the Carbon Concentration, Blend Concentration, and Renewal Rate in the Growth Kinetic ofChlorellasp.

Henrard

Rosa

Moraes

et al. 2014

The Scientific World Journal

View full text Add to dashboard Cite

The microalgae cultivation can be used as alternative sources of food, in agriculture, residual water treatment, and biofuels production. Semicontinuous cultivation is little studied but is more cost-effective than the discontinuous (batch) cultivation. In the semicontinuous cultivation, the microalga is maintained in better concentration of nutrients and the photoinhibition by excessive cell is reduced. Thus, biomass productivity and biocompounds of interest, such as lipid productivity, may be higher than in batch cultivation. The objective of this study was to examine the influence of blend concentration, medium renewal rate, and concentration of sodium bicarbonate on the growth of Chlorella sp. during semicontinuous cultivation. The cultivation was carried out in Raceway type bioreactors of 6 L, for 40 d at 30°C, 41.6 µmol m−2 s−1, and a 12 h light/dark photoperiod. Maximum specific growth rate (0.149 d−1) and generating biomass (2.89 g L−1) were obtained when the blend concentration was 0.80 g L−1, the medium renewal rate was 40%, and NaHCO3 was 1.60 g L−1. The average productivity (0.091 g L−1 d−1) was achieved with 0.8 g L−1 of blend concentration and NaHCO3 concentration of 1.6 g L−1, independent of the medium renewal rate.

show abstract

Optimal Renewal Rate and Nutrient Concentration for the Production of the Marine Microalga Phaeodactylum tricornutum in Semicontinuous Cultures

Cited by 31 publications

References 18 publications

Bacterial response to spatial gradients of algal-derived nutrients in a porous microplate

Bacterial response to spatial gradients of algal-derived nutrients in a porous microplate

Thermo‐alkaline pretreatment of lipid‐extracted microalgal biomass residues enhances hydrogen production

Effect of the Carbon Concentration, Blend Concentration, and Renewal Rate in the Growth Kinetic ofChlorellasp.

Contact Info

Product

Resources

About